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Assembly of combinatorial antibody libraries on phage surfaces - the gene-iii site

Academic Article
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Overview

authors

  • Barbas III, Carlos
  • Kang, A. S.
  • Lerner, Richard
  • Benkovic, S. J.

publication date

  • September 1991

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • A phagemid system was developed for the monovalent display of combinatorial antibody Fab libraries on the surface of filamentous phage M13. Fab fragments were fused to the carboxyl-terminal domain of the gene III protein. Phage displaying Fab fragments on their surface, or Phabs, were enriched by 10(3)- to 10(5)-fold on antigen-coated surfaces over nonspecific phage. The method may replace current antibody cloning techniques.

subject areas

  • Antibodies, Bacterial
  • Antibody Diversity
  • Base Sequence
  • Binding Sites, Antibody
  • Capsid
  • Cloning, Molecular
  • Gene Expression
  • Gene Library
  • Genetic Vectors
  • Immunoglobulin Fab Fragments
  • Molecular Sequence Data
  • Oligonucleotides
  • Recombinant Fusion Proteins
  • Tetanus Toxoid
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Research

keywords

  • CATALYTIC ANTIBODIES
  • COMBINATORIAL IMMUNOGLOBULIN REPERTOIRE
  • FILAMENTOUS PHAGE
  • HUMAN ANTIBODIES
  • SURFACE EXPRESSION
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Identity

PubMed Central ID

  • PMC52428

International Standard Serial Number (ISSN)

  • 0027-8424

PubMed ID

  • 1896445
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Additional Document Info

start page

  • 7978

end page

  • 7982

volume

  • 88

issue

  • 18

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