Improved sensitivity and coherence selection for n-15,h-1 -trosy elements in triple resonance experiments

Academic Article
uri icon

publication date

  • 1999

abstract

  • In experiments with proteins of molecular weights around 100 kDa the implementation of [15N,1H]-TROSY-elements in [15N]-constant-time triple resonance experiments yields sensitivity enhancements of one to two orders of magnitude. An additional gain of 10 to 20% may be obtained with the use of 'sensitivity enhancement elements'. This paper describes a novel sensitivity enhancement scheme which is based on concatenation of the 13C alpha-->15N magnetization transfer with the ST2-PT element, and which enables proper TROSY selection of the 15N multiplet components.