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Improved sensitivity and coherence selection for n-15,h-1 -trosy elements in triple resonance experiments

Academic Article
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Overview

authors

  • Salzmann, M.
  • Wider, G.
  • Pervushin, K.
  • Wuthrich, Kurt

publication date

  • October 1999

journal

  • Journal of Biomolecular NMR  Journal

abstract

  • In experiments with proteins of molecular weights around 100 kDa the implementation of [15N,1H]-TROSY-elements in [15N]-constant-time triple resonance experiments yields sensitivity enhancements of one to two orders of magnitude. An additional gain of 10 to 20% may be obtained with the use of 'sensitivity enhancement elements'. This paper describes a novel sensitivity enhancement scheme which is based on concatenation of the 13C alpha-->15N magnetization transfer with the ST2-PT element, and which enables proper TROSY selection of the 15N multiplet components.

subject areas

  • Aldehyde-Lyases
  • Bacterial Proteins
  • Magnetics
  • Nuclear Magnetic Resonance, Biomolecular
  • Sensitivity and Specificity
  • Staphylococcus aureus
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Research

keywords

  • TROSY in triple resonance experiments
  • concatenation
  • protein NMR
  • resonance assignments
  • sensitivity enhancement
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Identity

International Standard Serial Number (ISSN)

  • 0925-2738

Digital Object Identifier (DOI)

  • 10.1023/a:1008358030477

PubMed ID

  • 10605091
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Additional Document Info

start page

  • 181

end page

  • 184

volume

  • 15

issue

  • 2

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