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Transcription factor-IIIA induced bending of the Xenopus somatic 5S gene promoter

Academic Article
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Overview

authors

  • Schroth, G. P.
  • Cook, G. R.
  • Bradbury, E. M.
  • Gottesfeld, Joel

publication date

  • August 1989

journal

  • Nature  Journal

abstract

  • Transcription factor IIIA (TFIIIA), the canonical zinc-finger protein, is a protein of relative molecular mass 39,000 (39K) that is required for transcription of 5S-ribosomal subunit genes in Xenopus. It binds in a sequence-specific manner to the internal control region of the 5S gene (see Fig. 1) and facilitates transcription of the gene by RNA polymerase III. It also binds to the 5S gene product to form a 7S ribonucleoprotein particle. In oocytes the 7S particle acts as a storage form of the RNA to be utilized later in development. TFIIIA binds to DNA through its 30 K N-terminal domain, which contains nine zinc-fingers. TFIIIA was the first protein described to have this type of DNA binding motif, but numerous other proteins have now been shown to have zinc-finger domains. A structure for a single zinc-finger from the yeast protein ADR1, was recently proposed based on two-dimensional NMR data (ref. 8), and a similar structure was proposed based on comparison with crystal structures of other metalloproteins. Although models for the interaction of TFIIIA with the 5S-ribosomal gene DNA have been proposed, based on nuclease digestion and methylation interference data, little precise structural information is available for TFIIIA and the physical basis for the interaction of zinc-fingers with DNA is not understood. Using both circular permutation and circularization assays we provide convincing biochemical evidence that TFIIIA bends the DNA at the internal promoter of the 5S gene.

subject areas

  • Animals
  • DNA
  • Kinetics
  • Nucleic Acid Conformation
  • Promoter Regions, Genetic
  • RNA, Ribosomal
  • RNA, Ribosomal, 5S
  • Restriction Mapping
  • Transcription Factor TFIIIA
  • Transcription Factors
  • Xenopus
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Identity

International Standard Serial Number (ISSN)

  • 0028-0836

Digital Object Identifier (DOI)

  • 10.1038/340487a0

PubMed ID

  • 2755511
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Additional Document Info

start page

  • 487

end page

  • 488

volume

  • 340

issue

  • 6233

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