Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Inhibition of prolactin secretion by endothelin-3 is pertussis toxin-sensitive

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Burris, Thomas
  • Kanyicska, B.
  • Freeman, M. E.

publication date

  • 1991

journal

  • European Journal of Pharmacology  Journal

abstract

  • The effect of pertussis toxin (PTX) pretreatment on endothelin-3 (ET-3)-mediated inhibition of prolactin secretion from primary cultures of rat anterior pituitary cells was examined. Monolayer cultures of anterior pituitary cells were treated with either 20 ng/ml PTX dissolved in media or with media alone (control) on the third day of culture. Exactly 24 h after PTX pretreatment, cells were challenged with either 100 nM ET-3 dissolved in media or media alone (control) for 4 or 48 h. ET-3 significantly (P less than 0.01) inhibited prolactin secretion in both the 4 and 48 h incubations. However, if the cells had been previously treated with PTX, ET-3 did not significantly affect prolactin secretion. These data suggest that a PTX-sensitive G protein mediates ET-3-induced inhibition of prolactin secretion and that ET-3 may invoke a signal transduction mechanism in the lactotroph which is distinct from those described in other cell types.

subject areas

  • Animals
  • Cells, Cultured
  • Endothelins
  • Female
  • GTP-Binding Proteins
  • Pertussis Toxin
  • Pituitary Gland, Anterior
  • Prolactin
  • Radioimmunoassay
  • Rats
  • Signal Transduction
  • Virulence Factors, Bordetella
scroll to property group menus

Research

keywords

  • DOPAMINE
  • ENDOTHELIN
  • G-PROTEIN
  • PROLACTIN
  • SIGNAL TRANSDUCTION
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0014-2999

Digital Object Identifier (DOI)

  • 10.1016/0014-2999(91)90627-3

PubMed ID

  • 1907563
scroll to property group menus

Additional Document Info

start page

  • 223

end page

  • 225

volume

  • 198

issue

  • 2-3

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support