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An escherichia coli expression-based method for heme substitution

Academic Article
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Overview

authors

  • Woodward, J. J.
  • Martin, N. I.
  • Marletta, Michael

publication date

  • January 2007

journal

  • Nature Methods  Journal

abstract

  • Heme reconstitution with porphyrin analogs is a powerful approach toward understanding the molecular function of heme proteins; present methods, however, have not proven to be generally useful. Here we describe the development and application of an expression-based method for introducing modified porphyrins. The approach allows efficient incorporation of heme analogs using a widely available bacterial strain and offers an attractive alternative to present reconstitution methods that subject proteins to harsh, denaturing conditions.

subject areas

  • Chromatography, High Pressure Liquid
  • Escherichia coli
  • Hemeproteins
  • Porphyrins
  • Sensitivity and Specificity
  • Spectrophotometry, Ultraviolet
  • Time Factors
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Identity

International Standard Serial Number (ISSN)

  • 1548-7091

Digital Object Identifier (DOI)

  • 10.1038/nmeth984

PubMed ID

  • 17187078
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Additional Document Info

start page

  • 43

end page

  • 45

volume

  • 4

issue

  • 1

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