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Inflammatory microcrystals stimulate interleukin-6 production and secretion by human-monocytes and synoviocytes

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Overview

authors

  • Guerne, P. A.
  • Terkeltaub, R.
  • Zuraw, B.
  • Lotz, Martin

publication date

  • November 1989

journal

  • Arthritis and Rheumatism  Journal

abstract

  • Crystal-related joint diseases are often associated with systemic inflammatory manifestations, including increased levels of acute-phase proteins, leukocytosis, and fever. Recently, interleukin-6 (IL-6) has been identified as a pluripotent mediator of inflammatory and immunologic responses and the major hepatocyte-stimulating factor. In this study, we demonstrated that monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) crystals, and to a lesser extent, hydroxyapatite crystals, increased IL-6 production by synoviocytes and monocytes in vitro. Immunoprecipitation experiments showed that MSU and CPPD crystals, but not hydroxyapatite crystals, were able to increase the release of newly synthesized IL-6. Crystal-induced IL-6 stimulated acute-phase protein synthesis, immunoglobulin production, and hybridoma cell proliferation, which was neutralized by a specific antibody to IL-6. High levels of IL-6 were found in synovial fluid from patients with gout and pseudogout. These results demonstrate that MSU and CPPD crystals can induce IL-6 production in synoviocytes and monocytes, and that synovial fluid from patients with gout and pseudogout contains high levels of IL-6. Crystal-induced IL-6 is likely to be an important mediator of inflammatory responses in acute gout and pseudogout.

subject areas

  • Calcium Pyrophosphate
  • Cell Survival
  • Cells, Cultured
  • Chondrocalcinosis
  • Crystallization
  • Durapatite
  • Gout
  • Humans
  • Hydroxyapatites
  • Immunosorbent Techniques
  • Interleukin-1
  • Interleukin-6
  • Kinetics
  • Monocytes
  • Neutralization Tests
  • Synovial Fluid
  • Synovial Membrane
  • Uric Acid
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Identity

International Standard Serial Number (ISSN)

  • 0004-3591

Digital Object Identifier (DOI)

  • 10.1002/anr.1780321114

PubMed ID

  • 2554932
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Additional Document Info

start page

  • 1443

end page

  • 1452

volume

  • 32

issue

  • 11

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