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Differential infection of receptor-modified host-cells by receptor-specific influenza-viruses

Academic Article
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Overview

authors

  • Carroll, S. M.
  • Paulson, James

publication date

  • 1985

journal

  • Virus Research  Journal

abstract

  • Influenza viruses of contrasting receptor specificity have been examined for their ability to infect receptor-modified MDCK cells containing sialyloligosaccharide receptor determinants of defined sequence. Cells were treated with sialidase to remove sialic acid and render them resistant to infection and were then incubated with sialyltransferase and CMP-sialic acid to restore sialic acid in the SA alpha 2,6Gal or SA alpha 2,3Gal linkages. The viruses A/RI/5 + /57 and A/duck/Ukraine/1/63, previously shown to exhibit preferential binding of SA alpha 2,6Gal and SA alpha 2,3Gal linkages, respectively, were found to exhibit differential infection of the receptor-modified cells in accord with their receptor specificity. Coinfection of SA alpha 2,3Gal derivatized cells with a mixture of the two viruses resulted in selective propagation of the SA alpha 2,3Gal-specific A/duck/Ukraine/1/63 virus. The results demonstrate the potential for cell surface receptors to mediate selection of receptor-specific variants of influenza virus.

subject areas

  • Adsorption
  • Animals
  • Antibodies, Viral
  • Binding Sites
  • Cell Line
  • Dogs
  • Erythrocytes
  • Hemagglutinins, Viral
  • Influenza A virus
  • Kidney
  • Neuraminidase
  • Oligosaccharides
  • Receptors, Virus
  • Sialic Acids
  • Sialyltransferases
  • Species Specificity
  • Viral Proteins
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Identity

International Standard Serial Number (ISSN)

  • 0168-1702

Digital Object Identifier (DOI)

  • 10.1016/0168-1702(85)90006-1

PubMed ID

  • 4060886
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Additional Document Info

start page

  • 165

end page

  • 179

volume

  • 3

issue

  • 2

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