Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Expression of prepro-enkephalin in human articular chondrocytes is linked to cell-proliferation

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Villiger, P. M.
  • Lotz, Martin

publication date

  • January 1992

journal

  • EMBO Journal  Journal

abstract

  • This study shows that cultured human articular chondrocytes express high levels of 1.4 kb prepro-enkephalin mRNA. Chondrocytes store met-enkephalin intracellularly and secrete this neuropeptide in mature as well as in precursor form. Gene expression is inducible by serum factors. High levels of prepro-enkephalin mRNA are detected in proliferating chondrocytes but not in confluent, contact-inhibited cells. Phorbol myristate acetate and dibutyryl cyclic AMP, but not dexamethasone, increase levels of prepro-enkephalin mRNA. Furthermore, transforming growth factor beta (TGF beta) and platelet derived growth factor (PDGF) upregulate gene expression, whereas retinoic acid, which inhibits chondrocyte proliferation, suppresses both basal and induced gene expression. Using in situ hybridization it is shown that only 1-3% of primary chondrocytes express prepro-enkephalin mRNA, whereas 52 +/- 12% of subcultured cells are strongly positive. Analysis of DNA synthesis, by autoradiography of incorporated [3H]thymidine, shows that these numbers correspond to the percentage of cells in S-phase of the cell cycle. In cultures of primary chondrocytes TGF beta promotes the formation of cartilage nodules and stimulates proliferation of adherent cells. This is associated with high levels of prepro-enkephalin mRNA in proliferating cells but not in contact-inhibited cells in cartilage nodules. In contrast, formation of cartilage nodules, proliferation and the expression of enkephalin are suppressed by interleukin-1 beta. In summary, expression of prepro-enkephalin in human articular chondrocytes is differentially controlled by cartilage regulatory factors and closely associated with cell proliferation.

subject areas

  • Base Sequence
  • Bucladesine
  • Cartilage, Articular
  • Cell Division
  • Cells, Cultured
  • Dexamethasone
  • Enkephalin, Methionine
  • Enkephalins
  • Gene Expression Regulation
  • Growth Substances
  • Humans
  • Interleukin-1
  • Molecular Sequence Data
  • Protein Precursors
  • RNA, Messenger
  • Second Messenger Systems
  • Tetradecanoylphorbol Acetate
  • Tretinoin
  • Up-Regulation
scroll to property group menus

Research

keywords

  • CHONDROCYTES
  • ENKEPHALINS
  • GROWTH FACTORS
  • PROLIFERATION
  • RETINOIC ACID
scroll to property group menus

Identity

PubMed Central ID

  • PMC556434

International Standard Serial Number (ISSN)

  • 0261-4189

PubMed ID

  • 1310929
scroll to property group menus

Additional Document Info

start page

  • 135

end page

  • 143

volume

  • 11

issue

  • 1

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support