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Bulk segregation mapping of mutations in closely related strains of mice

Academic Article
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Overview

authors

  • Xia, Y.
  • Won, S.
  • Du, X.
  • Lin, P.
  • Ross, C.
  • La Vine, D.
  • Wiltshire, S.
  • Leiva, G.
  • Vidal, S. M.
  • Whittle, B.
  • Goodnow, C. C.
  • Koziol, James
  • Moresco, E. M. Y.
  • Beutler, Bruce

publication date

  • December 2010

journal

  • Genetics  Journal

abstract

  • Phenovariance may be obscured when genetic mapping is performed using highly divergent strains, and closely similar strains are preferred if adequate marker density can be established. We sequenced the C57BL/10J mouse genome using the Applied Biosystems SOLiD platform and here describe a genome-wide panel of informative markers that permits the mapping of mutations induced on the closely related C57BL/6J background by outcrossing to C57BL/10J, and backcrossing or intercrossing. The panel consists of 127 single nucleotide polymorphisms validated by capillary sequencing: 124 spaced at ∼20-Mb intervals across the 19 autosomes, and three markers on the X chromosome. We determined the genetic relationship between four C57BL-derived substrains and used the panel to map two N-ethyl-N-nitrosourea (ENU)-induced mutations responsible for visible phenotypes in C57BL/6J mice through bulk segregation analysis. Capillary sequencing, with computation of relative chromatogram peak heights, was used to determine the proportion of alleles from each strain at each marker.

subject areas

  • Alleles
  • Animals
  • Chromosomes
  • Genetic Markers
  • Genome
  • Genomics
  • Mice
  • Mice, Inbred Strains
  • Mutagenesis
  • Mutation
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA
  • Species Specificity
  • X Chromosome
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Identity

PubMed Central ID

  • PMC2998299

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1534/genetics.110.121160

PubMed ID

  • 20923982
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Additional Document Info

start page

  • 1139

end page

  • 1146

volume

  • 186

issue

  • 4

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