recent publications
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academic article
- Miyamori, T., Nakasone, Y., Hitomi, K., Christie, J. M., Getzoff, E. D., Terazima, M. Reaction dynamics of the UV-B photosensor UVR8 Photochemical & Photobiological Sciences 2015 14:995-1004 DOI:10.1039/c5pp00012b PMID:25811405
- Wijaya, I. M. M., Iwata, T., Yamamoto, J., Hitomi, K., Iwai, S., Getzoff, E. D., Kennis, J. T. M., Mathes, T., Kandori, H. Flavin adenine dinucleotide chromophore charge controls the conformation of cyclobutane pyrimidine dimer photolyase alpha-helices Biochemistry 2014 53:5864-5875 DOI:10.1021/bi500638b PMID:25152314
- Muller, P., Bouly, J. P., Hitomi, K., Balland, V., Getzoff, E. D., Ritz, T., Brettel, K. ATP binding turns plant cryptochrome into an efficient natural photoswitch Scientific Reports 2014 4:5175 DOI:10.1038/srep05175 PMID:24898692 PMCID:PMC4046262
- Waadt, R., Hitomi, K., Nishimura, N., Hitomi, C., Adams, S. R., Getzoff, E. D., Schroeder, J. I. FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis Elife 2014 3:e01739 DOI:10.7554/eLife.01739 PMID:24737861 PMCID:PMC3985518
- Hitomi, K., Getzoff, E. D., Schroeder, J. I. Going green: phytohormone mimetics for drought rescue Plant Physiology 2013 163:1087-1088 DOI:10.1104/pp.113.227660 PMID:24172784 PMCID:PMC3813634
- Biskup, T., Paulus, B., Okafuji, A., Hitomi, K., Getzoff, E. D., Weber, S., Schleicher, E. Variable electron transfer pathways in an amphibian cryptochrome: Tryptophan versus tyrosine-based radical pairs Journal of Biological Chemistry 2013 288:9249-9260 DOI:10.1074/jbc.M112.417725 PMID:23430261 PMCID:PMC3610996
- Tsutakawa, S. E., Shin, D. S., Mol, C. D., Izumi, T., Arvai, A. S., Mantha, A. K., Szczesny, B., Ivanov, I. N., Hosfield, D. J., Maiti, B., Pique, M. E., Frankel, K. A., et al. Conserved structural chemistry for incision activity in structurally non-homologous apurinic/apyrimidinic endonuclease APE1 and endonuclease IV DNA repair enzymes Journal of Biological Chemistry 2013 288:8445-8455 DOI:10.1074/jbc.M112.422774 PMID:23355472 PMCID:PMC3605660
- Wijaya, I. M. M., Zhang, Y., Iwata, T., Yamamoto, J., Hitomi, K., Iwai, S., Getzoff, E. D., Kandori, H. Detection of distinct α-helical rearrangements of cyclobutane pyrimidine dimer photolyase upon substrate binding by Fourier transform infrared spectroscopy Biochemistry 2013 52:1019-1027 DOI:10.1021/bi3016179 PMID:23331252
- Yamada, D., Zhang, Y., Iwata, T., Hitomi, K., Getzoff, E. D., Kandori, H. Fourier-transform infrared study of the photoactivation process of xenopus (6-4) photolyase Biochemistry 2012 51:5774-5783 DOI:10.1021/bi300530x PMID:22747528 PMCID:PMC4329314
- Christie, J. M., Hitomi, K., Arvai, A. S., Hartfield, K. A., Mettlen, M., Pratt, A. J., Tainer, J. A., Getzoff, E. D. Structural tuning of the fluorescent protein iLOV for improved photostability Journal of Biological Chemistry 2012 287:22295-22304 DOI:10.1074/jbc.M111.318881 PMID:22573334 PMCID:PMC3381190
- Hitomi, K., Arvai, A. S., Yamamoto, J., Hitomi, C., Teranishi, M., Hirouchi, T., Yamamoto, K., Iwai, S., Tainer, J. A., Hidema, J., Getzoff, E. D. Eukaryotic class II cyclobutane pyrimidine dimer photolyase structure reveals basis for improved ultraviolet tolerance in plants Journal of Biological Chemistry 2012 287:12060-12069 DOI:10.1074/jbc.M111.244020 PMID:22170053 PMCID:PMC3320952
- Christie, J. M., Arvai, A. S., Baxter, K. J., Heilmann, M., Pratt, A. J., O'Hara, A., Kelly, S. M., Hothorn, M., Smith, B. O., Hitomi, K., Jenkins, G. I., Getzoff, E. D. Plant UVR8 photoreceptor senses UV-B by tryptophan-mediated disruption of cross-dimer salt bridges Science 2012 335:1492-1496 DOI:10.1126/science.1218091 PMID:22323738 PMCID:PMC3505452
- Zhang, Y., Yamamoto, J., Yamada, D., Iwata, T., Hitomi, K., Todo, T., Getzoff, E. D., Iwai, S., Kandorit, H. Substrate assignment of the (6-4) photolyase reaction by FTIR spectroscopy Journal of Physical Chemistry Letters 2011 2:2774-2777 DOI:10.1021/jz201250b
- Kondoh, M., Shiraishi, C., Muller, P., Ahmad, M., Hitomi, K., Getzoff, E. D., Terazima, M. Light-induced conformational changes in full-length Arabidopsis thaliana cryptochrome Journal of Molecular Biology 2011 413:128-137 DOI:10.1016/j.jmb.2011.08.031 PMID:21875594 PMCID:PMC4451184
- Zhang, Y., Iwata, T., Yamamoto, J., Hitomi, K., Iwai, S., Todo, T., Getzoff, E. D., Kandori, H. FTIR study of light-dependent activation and DNA repair processes of (6-4) photolyase Biochemistry 2011 50:3591-3598 DOI:10.1021/bi1019397 PMID:21462921
- Kondoh, M., Hitomi, K., Yamamoto, J., Todo, T., Iwai, S., Getzoff, E. D., Terazima, M. Light-induced conformational change and product release in DNA repair by (6-4) photolyase Journal of the American Chemical Society 2011 133:2183-2191 DOI:10.1021/ja107691w PMID:21271694 PMCID:PMC4342989
- Biskup, T., Hitomi, K., Getzoff, E. D., Krapf, S., Koslowski, T., Schleicher, E., Weber, S. Unexpected electron transfer in cryptochrome identified by time-resolved EPR spectroscopy Angewandte Chemie-International Edition 2011 50:12647-12651 DOI:10.1002/anie.201104321 PMID:22086606 PMCID:PMC3517006
- Weber, S., Biskup, T., Okafuji, A., Marino, A. R., Berthold, T., Link, G., Hitomi, K., Getzoff, E. D., Schleicher, E., Norris, J. R. Origin of light-induced spin-correlated radical pairs in cryptochrome Journal of Physical Chemistry B 2010 114:14745-14754 DOI:10.1021/jp103401u PMID:20684534 PMCID:PMC4329313
- Iwata, T., Zhang, Y., Hitomi, K., Getzoff, E. D., Kandori, H. Key dynamics of conserved asparagine in a cryptochrome/photolyase family protein by fourier transform infrared spectroscopy Biochemistry 2010 49:8882-8891 DOI:10.1021/bi1009979 PMID:20828134 PMCID:PMC4329311
- Hubbard, K. E., Nishimura, N., Hitomi, K., Getzoff, E. D., Schroeder, J. I. Early abscisic acid signal transduction mechanisms: Newly discovered components and newly emerging questions Genes & Development 2010 24:1695-1708 DOI:10.1101/gad.1953910 PMID:20713515 PMCID:PMC2922499
- Okafuji, A., Biskup, T., Hitomi, K., Getzoff, E. D., Kaiser, G., Batschauer, A., Bacher, A., Hidema, J., Teranishi, M., Yamamoto, K., Schleicher, E., Weber, S. Light-induced activation of class II cyclobutane pyrimidine dimer photolyases DNA Repair 2010 9:495-505 DOI:10.1016/j.dnarep.2010.01.014 PMID:20227927
- Schleicher, E., Wenzel, R., Ahmad, M., Batschauer, A., Essen, L. O., Hitomi, K., Getzoff, E. D., Bittl, R., Weber, S., Okafuji, A. The electronic state of flavoproteins: Investigations with proton electron-nuclear double resonance Applied Magnetic Resonance 2010 37:339-352 DOI:10.1007/s00723-009-0101-8 PMID:26089595 PMCID:PMC4469238
- Nishimura, N., Hitomi, K., Arvai, A. S., Rambo, R. P., Hitomi, C., Cutler, S. R., Schroeder, J. I., Getzoff, E. D. Structural mechanism of abscisic acid binding and signaling by dimeric PYR1 Science 2009 326:1373-1379 DOI:10.1126/science.1181829 PMID:19933100 PMCID:PMC2835493
- Yamamoto, J., Hitomi, K., Hayashi, R., Getzoff, E. D., Iwai, S. Role of the carbonyl group of the (6-4) photoproduct in the (6-4) photolyase reaction Biochemistry 2009 48:9306-9312 DOI:10.1021/bi900956p PMID:19715341 PMCID:PMC2777982
- Hitomi, K., DiTacchio, L., Arvai, A. S., Yamamoto, J., Kim, S. T., Todo, T., Tainer, J. A., Iwai, S., Panda, S., Getzoff, E. D. Functional motifs in the (6-4) photolyase crystal structure make a comparative framework for DNA repair photolyases and clock cryptochromes Proceedings of the National Academy of Sciences of the United States of America 2009 106:6962-6967 DOI:10.1073/pnas.0809180106 PMID:19359474 PMCID:PMC2678464
- Biskup, T., Schleicher, E., Okafuji, A., Link, G., Hitomi, K., Getzoff, E. D., Weber, S. Direct observation of a photoinduced radical pair in a cryptochrome blue-light photoreceptor Angewandte Chemie-International Edition 2009 48:404-407 DOI:10.1002/anie.200803102 PMID:19058271 PMCID:PMC4329312
- Yamamoto, J., Hitomi, K., Hayashi, R., Getzoff, E. D., Iwai, S. Recognition and reaction mechanisms of the (6-4) photolyase as determined by using a (6-4) photoproduct analog Nucleic Acids Symposium Series 2009 221-222 DOI:10.1093/nass/nrp111 PMID:19749340
- Hitomi, K., Iwai, S., Tainer, J. A. The intricate structural chemistry of base excision repair machinery: Implications for DNA damage recognition, removal, and repair DNA Repair 2007 6:410-428 DOI:10.1016/j.dnarep.2006.10.004 PMID:17208522
- Schleicher, E., Hitomi, K., Kay, C. W. M., Getzoff, E. D., Todo, T., Weber, S. Electron nuclear double resonance differentiates complementary roles for active site histidines in (6-4) photolyase Journal of Biological Chemistry 2007 282:4738-4747 DOI:10.1074/jbc.M604734200 PMID:17164245
- Doi, Y., Katafuchi, A., Fujiwara, Y., Hitomi, K., Tainer, J. A., Ide, H., Iwai, S. Synthesis and characterization of oligonucleotides containing 2 '-fluorinated thymidine glycol as inhibitors of the endonuclease iii reaction Nucleic Acids Research 2006 34:1540-1551 DOI:10.1093/nar/gkl061 PMID:16547199 PMCID:PMC1409675
- Hitomi, K., Oyama, T., Han, S. G., Arvai, A. S., Getzoff, E. D. Tetrameric architecture of the circadian clock protein KaiB - a novel interface for intermolecular interactions and its impact on the circadian rhythm Journal of Biological Chemistry 2005 280:19127-19135 DOI:10.1074/jbc.M411284200 PMID:15716274
- Manuel, R. C., Hitomi, K., Arvai, A. S., House, P. G., Kurtz, A. J., Dodson, M. L., McCullough, A. K., Tainer, J. A., Lloyd, R. S. Reaction intermediates in the catalytic mechanism of escherichia coli muty DNA glycosylase Journal of Biological Chemistry 2004 279:46930-46939 DOI:10.1074/jbc.M403944200 PMID:15326180
- Brudler, R., Hitomi, K., Daiyasu, H., Toh, H., Kucho, K., Ishiura, M., Kanehisa, M., Roberts, V. A., Todo, T., Tainer, J. A., Getzoff, E. D. Identification of a new cryptochrome class: Structure, function, and evolution Molecular Cell 2003 11:59-67 DOI:10.1016/s1097-2765(03)00008-x PMID:12535521