recent publications

• academic article

  • Miyamori, T., Nakasone, Y., Hitomi, K., Christie, J. M., Getzoff, E. D., Terazima, M. Reaction dynamics of the UV-B photosensor UVR8 Photochemical & Photobiological Sciences  2015 14:995-1004  DOI:10.1039/c5pp00012b  PMID:25811405
  • Wijaya, I. M. M., Iwata, T., Yamamoto, J., Hitomi, K., Iwai, S., Getzoff, E. D., Kennis, J. T. M., Mathes, T., Kandori, H. Flavin adenine dinucleotide chromophore charge controls the conformation of cyclobutane pyrimidine dimer photolyase alpha-helices Biochemistry  2014 53:5864-5875  DOI:10.1021/bi500638b  PMID:25152314
  • Muller, P., Bouly, J. P., Hitomi, K., Balland, V., Getzoff, E. D., Ritz, T., Brettel, K. ATP binding turns plant cryptochrome into an efficient natural photoswitch Scientific Reports  2014 4:5175  DOI:10.1038/srep05175  PMID:24898692  PMCID:PMC4046262
  • Waadt, R., Hitomi, K., Nishimura, N., Hitomi, C., Adams, S. R., Getzoff, E. D., Schroeder, J. I. FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis Elife  2014 3:e01739  DOI:10.7554/eLife.01739  PMID:24737861  PMCID:PMC3985518
  • Hitomi, K., Getzoff, E. D., Schroeder, J. I. Going green: phytohormone mimetics for drought rescue Plant Physiology  2013 163:1087-1088  DOI:10.1104/pp.113.227660  PMID:24172784  PMCID:PMC3813634
  • Biskup, T., Paulus, B., Okafuji, A., Hitomi, K., Getzoff, E. D., Weber, S., Schleicher, E. Variable electron transfer pathways in an amphibian cryptochrome: Tryptophan versus tyrosine-based radical pairs Journal of Biological Chemistry  2013 288:9249-9260  DOI:10.1074/jbc.M112.417725  PMID:23430261  PMCID:PMC3610996
  • Tsutakawa, S. E., Shin, D. S., Mol, C. D., Izumi, T., Arvai, A. S., Mantha, A. K., Szczesny, B., Ivanov, I. N., Hosfield, D. J., Maiti, B., Pique, M. E., Frankel, K. A., et al. Conserved structural chemistry for incision activity in structurally non-homologous apurinic/apyrimidinic endonuclease APE1 and endonuclease IV DNA repair enzymes Journal of Biological Chemistry  2013 288:8445-8455  DOI:10.1074/jbc.M112.422774  PMID:23355472  PMCID:PMC3605660
  • Wijaya, I. M. M., Zhang, Y., Iwata, T., Yamamoto, J., Hitomi, K., Iwai, S., Getzoff, E. D., Kandori, H. Detection of distinct α-helical rearrangements of cyclobutane pyrimidine dimer photolyase upon substrate binding by Fourier transform infrared spectroscopy Biochemistry  2013 52:1019-1027  DOI:10.1021/bi3016179  PMID:23331252
  • Yamada, D., Zhang, Y., Iwata, T., Hitomi, K., Getzoff, E. D., Kandori, H. Fourier-transform infrared study of the photoactivation process of xenopus (6-4) photolyase Biochemistry  2012 51:5774-5783  DOI:10.1021/bi300530x  PMID:22747528  PMCID:PMC4329314
  • Christie, J. M., Hitomi, K., Arvai, A. S., Hartfield, K. A., Mettlen, M., Pratt, A. J., Tainer, J. A., Getzoff, E. D. Structural tuning of the fluorescent protein iLOV for improved photostability Journal of Biological Chemistry  2012 287:22295-22304  DOI:10.1074/jbc.M111.318881  PMID:22573334  PMCID:PMC3381190
  • Hitomi, K., Arvai, A. S., Yamamoto, J., Hitomi, C., Teranishi, M., Hirouchi, T., Yamamoto, K., Iwai, S., Tainer, J. A., Hidema, J., Getzoff, E. D. Eukaryotic class II cyclobutane pyrimidine dimer photolyase structure reveals basis for improved ultraviolet tolerance in plants Journal of Biological Chemistry  2012 287:12060-12069  DOI:10.1074/jbc.M111.244020  PMID:22170053  PMCID:PMC3320952
  • Christie, J. M., Arvai, A. S., Baxter, K. J., Heilmann, M., Pratt, A. J., O'Hara, A., Kelly, S. M., Hothorn, M., Smith, B. O., Hitomi, K., Jenkins, G. I., Getzoff, E. D. Plant UVR8 photoreceptor senses UV-B by tryptophan-mediated disruption of cross-dimer salt bridges Science  2012 335:1492-1496  DOI:10.1126/science.1218091  PMID:22323738  PMCID:PMC3505452
  • Zhang, Y., Yamamoto, J., Yamada, D., Iwata, T., Hitomi, K., Todo, T., Getzoff, E. D., Iwai, S., Kandorit, H. Substrate assignment of the (6-4) photolyase reaction by FTIR spectroscopy Journal of Physical Chemistry Letters  2011 2:2774-2777  DOI:10.1021/jz201250b
  • Kondoh, M., Shiraishi, C., Muller, P., Ahmad, M., Hitomi, K., Getzoff, E. D., Terazima, M. Light-induced conformational changes in full-length Arabidopsis thaliana cryptochrome Journal of Molecular Biology  2011 413:128-137  DOI:10.1016/j.jmb.2011.08.031  PMID:21875594  PMCID:PMC4451184
  • Zhang, Y., Iwata, T., Yamamoto, J., Hitomi, K., Iwai, S., Todo, T., Getzoff, E. D., Kandori, H. FTIR study of light-dependent activation and DNA repair processes of (6-4) photolyase Biochemistry  2011 50:3591-3598  DOI:10.1021/bi1019397  PMID:21462921
  • Kondoh, M., Hitomi, K., Yamamoto, J., Todo, T., Iwai, S., Getzoff, E. D., Terazima, M. Light-induced conformational change and product release in DNA repair by (6-4) photolyase Journal of the American Chemical Society  2011 133:2183-2191  DOI:10.1021/ja107691w  PMID:21271694  PMCID:PMC4342989
  • Biskup, T., Hitomi, K., Getzoff, E. D., Krapf, S., Koslowski, T., Schleicher, E., Weber, S. Unexpected electron transfer in cryptochrome identified by time-resolved EPR spectroscopy Angewandte Chemie-International Edition  2011 50:12647-12651  DOI:10.1002/anie.201104321  PMID:22086606  PMCID:PMC3517006
  • Weber, S., Biskup, T., Okafuji, A., Marino, A. R., Berthold, T., Link, G., Hitomi, K., Getzoff, E. D., Schleicher, E., Norris, J. R. Origin of light-induced spin-correlated radical pairs in cryptochrome Journal of Physical Chemistry B  2010 114:14745-14754  DOI:10.1021/jp103401u  PMID:20684534  PMCID:PMC4329313
  • Iwata, T., Zhang, Y., Hitomi, K., Getzoff, E. D., Kandori, H. Key dynamics of conserved asparagine in a cryptochrome/photolyase family protein by fourier transform infrared spectroscopy Biochemistry  2010 49:8882-8891  DOI:10.1021/bi1009979  PMID:20828134  PMCID:PMC4329311
  • Hubbard, K. E., Nishimura, N., Hitomi, K., Getzoff, E. D., Schroeder, J. I. Early abscisic acid signal transduction mechanisms: Newly discovered components and newly emerging questions Genes & Development  2010 24:1695-1708  DOI:10.1101/gad.1953910  PMID:20713515  PMCID:PMC2922499
  • Okafuji, A., Biskup, T., Hitomi, K., Getzoff, E. D., Kaiser, G., Batschauer, A., Bacher, A., Hidema, J., Teranishi, M., Yamamoto, K., Schleicher, E., Weber, S. Light-induced activation of class II cyclobutane pyrimidine dimer photolyases DNA Repair  2010 9:495-505  DOI:10.1016/j.dnarep.2010.01.014  PMID:20227927
  • Schleicher, E., Wenzel, R., Ahmad, M., Batschauer, A., Essen, L. O., Hitomi, K., Getzoff, E. D., Bittl, R., Weber, S., Okafuji, A. The electronic state of flavoproteins: Investigations with proton electron-nuclear double resonance Applied Magnetic Resonance  2010 37:339-352  DOI:10.1007/s00723-009-0101-8  PMID:26089595  PMCID:PMC4469238
  • Nishimura, N., Hitomi, K., Arvai, A. S., Rambo, R. P., Hitomi, C., Cutler, S. R., Schroeder, J. I., Getzoff, E. D. Structural mechanism of abscisic acid binding and signaling by dimeric PYR1 Science  2009 326:1373-1379  DOI:10.1126/science.1181829  PMID:19933100  PMCID:PMC2835493
  • Yamamoto, J., Hitomi, K., Hayashi, R., Getzoff, E. D., Iwai, S. Role of the carbonyl group of the (6-4) photoproduct in the (6-4) photolyase reaction Biochemistry  2009 48:9306-9312  DOI:10.1021/bi900956p  PMID:19715341  PMCID:PMC2777982
  • Hitomi, K., DiTacchio, L., Arvai, A. S., Yamamoto, J., Kim, S. T., Todo, T., Tainer, J. A., Iwai, S., Panda, S., Getzoff, E. D. Functional motifs in the (6-4) photolyase crystal structure make a comparative framework for DNA repair photolyases and clock cryptochromes Proceedings of the National Academy of Sciences of the United States of America  2009 106:6962-6967  DOI:10.1073/pnas.0809180106  PMID:19359474  PMCID:PMC2678464
  • Biskup, T., Schleicher, E., Okafuji, A., Link, G., Hitomi, K., Getzoff, E. D., Weber, S. Direct observation of a photoinduced radical pair in a cryptochrome blue-light photoreceptor Angewandte Chemie-International Edition  2009 48:404-407  DOI:10.1002/anie.200803102  PMID:19058271  PMCID:PMC4329312
  • Yamamoto, J., Hitomi, K., Hayashi, R., Getzoff, E. D., Iwai, S. Recognition and reaction mechanisms of the (6-4) photolyase as determined by using a (6-4) photoproduct analog Nucleic Acids Symposium Series  2009 221-222  DOI:10.1093/nass/nrp111  PMID:19749340
  • Hitomi, K., Iwai, S., Tainer, J. A. The intricate structural chemistry of base excision repair machinery: Implications for DNA damage recognition, removal, and repair DNA Repair  2007 6:410-428  DOI:10.1016/j.dnarep.2006.10.004  PMID:17208522
  • Schleicher, E., Hitomi, K., Kay, C. W. M., Getzoff, E. D., Todo, T., Weber, S. Electron nuclear double resonance differentiates complementary roles for active site histidines in (6-4) photolyase Journal of Biological Chemistry  2007 282:4738-4747  DOI:10.1074/jbc.M604734200  PMID:17164245
  • Doi, Y., Katafuchi, A., Fujiwara, Y., Hitomi, K., Tainer, J. A., Ide, H., Iwai, S. Synthesis and characterization of oligonucleotides containing 2 '-fluorinated thymidine glycol as inhibitors of the endonuclease iii reaction Nucleic Acids Research  2006 34:1540-1551  DOI:10.1093/nar/gkl061  PMID:16547199  PMCID:PMC1409675
  • Hitomi, K., Oyama, T., Han, S. G., Arvai, A. S., Getzoff, E. D. Tetrameric architecture of the circadian clock protein KaiB - a novel interface for intermolecular interactions and its impact on the circadian rhythm Journal of Biological Chemistry  2005 280:19127-19135  DOI:10.1074/jbc.M411284200  PMID:15716274
  • Manuel, R. C., Hitomi, K., Arvai, A. S., House, P. G., Kurtz, A. J., Dodson, M. L., McCullough, A. K., Tainer, J. A., Lloyd, R. S. Reaction intermediates in the catalytic mechanism of escherichia coli muty DNA glycosylase Journal of Biological Chemistry  2004 279:46930-46939  DOI:10.1074/jbc.M403944200  PMID:15326180
  • Brudler, R., Hitomi, K., Daiyasu, H., Toh, H., Kucho, K., Ishiura, M., Kanehisa, M., Roberts, V. A., Todo, T., Tainer, J. A., Getzoff, E. D. Identification of a new cryptochrome class: Structure, function, and evolution Molecular Cell  2003 11:59-67  DOI:10.1016/s1097-2765(03)00008-x  PMID:12535521