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Direct allelic variation scanning of the yeast genome

Academic Article
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Overview

authors

  • Winzeler, Elizabeth
  • Richards, D. R.
  • Conway, A. R.
  • Goldstein, A. L.
  • Kalman, S.
  • McCullough, M. J.
  • McCusker, J. H.
  • Stevens, D. A.
  • Wodicka, L.
  • Lockhart, D. J.
  • Davis, R. W.

publication date

  • August 1998

journal

  • Science  Journal

abstract

  • As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction, without creating new strains or constructs, and without knowing the specific nature of the variation. A total of 3714 biallelic markers, spaced about every 3.5 kilobases, were identified by analyzing the patterns obtained when total genomic DNA from two different strains of yeast was hybridized to high-density oligonucleotide arrays. The markers were then used to simultaneously map a multidrug-resistance locus and four other loci with high resolution (11 to 64 kilobases).

subject areas

  • Alleles
  • Chromosome Mapping
  • Cycloheximide
  • Drug Resistance, Microbial
  • Drug Resistance, Multiple
  • Gene Deletion
  • Genes, Fungal
  • Genetic Linkage
  • Genetic Markers
  • Genetic Techniques
  • Genetic Variation
  • Genome, Fungal
  • Genotype
  • Nucleic Acid Hybridization
  • Phenotype
  • Recombination, Genetic
  • Saccharomyces cerevisiae
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.281.5380.1194

PubMed ID

  • 9712584
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Additional Document Info

start page

  • 1194

end page

  • 1197

volume

  • 281

issue

  • 5380

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