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Direct analysis of protein mixtures by tandem mass spectrometry

Academic Article
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Overview

authors

  • Yates III, John
  • McCormack, A. L.
  • Schieltz, D.
  • Carmack, E.
  • Link, A.

publication date

  • July 1997

journal

  • Journal of Protein Chemistry  Journal

abstract

  • Methods to identify proteins contained in mixtures are described. The approach uses microcolumn liquid chromatography and automated tandem mass spectrometry in conjunction with protein and nucleotide database searching algorithms. This approach is applied to the identification of proteins obtained by immunoprecipitation reactions, interaction with a GST protein fusion products and interaction with a macromolecular complex.

subject areas

  • Chromatography, Liquid
  • Databases, Factual
  • Macromolecular Substances
  • Mass Spectrometry
  • Precipitin Tests
  • Proteins
  • Sensitivity and Specificity
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Research

keywords

  • SEQUEST
  • database searching
  • multiprotein complexes
  • tandem mass spectrometry
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Identity

International Standard Serial Number (ISSN)

  • 0277-8033

Digital Object Identifier (DOI)

  • 10.1023/A:1026365528484

PubMed ID

  • 9246634
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Additional Document Info

start page

  • 495

end page

  • 497

volume

  • 16

issue

  • 5

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