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The C-terminal region of lymphocytic choriomeningitis virus nucleoprotein contains distinct and segregable functional domains involved in NP-Z interaction and counteraction of the type I interferon response

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Overview

authors

  • Ortiz-Riano, E.
  • Cheng, B. Y. H.
  • de la Torre, Juan
  • Martinez-Sobrido, L.

publication date

  • December 2011

journal

  • Journal of Virology  Journal

abstract

  • Several arenaviruses cause hemorrhagic fever (HF) disease in humans that is associated with high morbidity and significant mortality. Arenavirus nucleoprotein (NP), the most abundant viral protein in infected cells and virions, encapsidates the viral genome RNA, and this NP-RNA complex, together with the viral L polymerase, forms the viral ribonucleoprotein (vRNP) that directs viral RNA replication and gene transcription. Formation of infectious arenavirus progeny requires packaging of vRNPs into budding particles, a process in which arenavirus matrix-like protein (Z) plays a central role. In the present study, we have characterized the NP-Z interaction for the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV). The LCMV NP domain that interacted with Z overlapped with a previously documented C-terminal domain that counteracts the host type I interferon (IFN) response. However, we found that single amino acid mutations that affect the anti-IFN function of LCMV NP did not disrupt the NP-Z interaction, suggesting that within the C-terminal region of NP different amino acid residues critically contribute to these two distinct and segregable NP functions. A similar NP-Z interaction was confirmed for the HF arenavirus Lassa virus (LASV). Notably, LCMV NP interacted similarly with both LCMV Z and LASV Z, while LASV NP interacted only with LASV Z. Our results also suggest the presence of a conserved protein domain within NP but with specific amino acid residues playing key roles in determining the specificity of NP-Z interaction that may influence the viability of reassortant arenaviruses. In addition, this NP-Z interaction represents a potential target for the development of antiviral drugs to combat human-pathogenic arenaviruses.

subject areas

  • Amino Acid Substitution
  • Animals
  • Carrier Proteins
  • Cell Line
  • Interferon Type I
  • Lassa virus
  • Lymphocytic choriomeningitis virus
  • Mutagenesis, Site-Directed
  • Mutant Proteins
  • Nucleoproteins
  • Point Mutation
  • Protein Interaction Domains and Motifs
  • Protein Interaction Mapping
  • Virus Assembly
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Identity

PubMed Central ID

  • PMC3233175

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.05834-11

PubMed ID

  • 21976642
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Additional Document Info

start page

  • 13038

end page

  • 13048

volume

  • 85

issue

  • 24

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