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Platelet-activating factor stimulates transcription of the heparin-binding epidermal growth factor-like growth factor in monocytes. Correlation with an increased kappa B binding activity

Academic Article
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Overview

authors

  • Pan, Z. X.
  • Kravchenko, Vladimir
  • Ye, R. D.

publication date

  • April 1995

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Human peripheral blood monocytes responded to stimulation of platelet-activating factor (PAF) with up-regulation of the transcript for heparin-binding epidermal growth factor-like growth factor (HB-EGF), a potent mitogen for vascular smooth muscle cells. This function of PAF was observed at nanomolar concentrations of the ligand, starting at 30 min after stimulation. The PAF-induced up-regulation of HB-EGF mRNA was accompanied by an increase in kappa B binding activity. These functions of PAF appeared to be mediated through the cell surface PAF receptors, as two PAF receptor antagonists, WEB 2086 and L-659,989, blocked both the up-regulation of HB-EGF mRNA and kappa B binding activity induced by PAF. The antagonists, however, had no effect on phorbol ester-induced up-regulation of HB-EGF mRNA and kappa B binding activity. Pretreatment of monocytes with pertussis toxin inhibited these functions of PAF, whereas cholera toxin had no inhibitory effect. Pyrrolidine dithiocarbamate, an inhibitor for NF-kappa B activation, markedly reduced PAF-stimulated kappa B binding activity as well as up-regulation of HB-EGF mRNA. These results suggest a potential role of PAF in HB-EGF expression and provide evidence that this stimulation may occur through increased kappa B binding activity.

subject areas

  • Bacterial Toxins
  • Base Sequence
  • DNA Primers
  • Epidermal Growth Factor
  • Gene Expression Regulation
  • Heparin
  • Heparin-binding EGF-like Growth Factor
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Molecular Sequence Data
  • Monocytes
  • NF-kappa B
  • Platelet Activating Factor
  • Platelet Membrane Glycoproteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Transcription, Genetic
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 7713868
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Additional Document Info

start page

  • 7787

end page

  • 7790

volume

  • 270

issue

  • 14

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