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A t-cell receptor-v-alpha region selectively expressed in cd4+ cells

Academic Article
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Overview

authors

  • Jameson, S. C.
  • Kaye, J.
  • Gascoigne, Nicholas

publication date

  • September 1990

journal

  • Journal of Immunology  Journal

abstract

  • The peripheral TCR V beta repertoire is strongly influenced by the processes of negative selection (deletion) and positive selection in the thymus. In order to investigate whether such selection events influence the V alpha repertoire, we have produced an anti-V alpha 11 mAb. This antibody was made by immunization with a chimeric TCR:Ig protein containing V alpha 11 in place of the VH of an IgG2a, lambda Ig. This scheme optimizes the specificity of immunization and facilitates the screening procedure. The antibody recognizes a panel of V alpha 11-expressing T cell clones. Analysis of mouse strains indicates that the antibody recognizes V alpha 11 only in mice of the C57 background. The expression of the epitope on peripheral T cells is strongly biased to the CD4+ subset, suggesting positive selection of V alpha 11 on class II MHC molecules. In some strain comparisons, the percentage of V alpha 11-expressing T cells in the CD4+ subset was elevated in I-E+ relative to I-E- strains. These data suggest that V alpha 11 can differentially influence the selection of T cells into the CD4+/CD8+ subsets.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Surface
  • CD4-Positive T-Lymphocytes
  • Epitopes
  • Flow Cytometry
  • Gene Expression
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Genes, Dominant
  • Histocompatibility Antigens Class II
  • Mice
  • Mice, Inbred C57BL
  • Minor Lymphocyte Stimulatory Antigens
  • Precipitin Tests
  • Receptors, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 1696594
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Additional Document Info

start page

  • 1324

end page

  • 1331

volume

  • 145

issue

  • 5

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