Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Local clustering of transferrin receptors promotes clathrin-coated pit initiation

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Liu, A. P.
  • Aguet, F.
  • Danuser, G.
  • Schmid, Sandra

publication date

  • December 2010

journal

  • Journal of Cell Biology  Journal

abstract

  • Clathrin-mediated endocytosis (CME) is the major pathway for concentrative uptake of receptors and receptor-ligand complexes (cargo). Although constitutively internalized cargos are known to accumulate into maturing clathrin-coated pits (CCPs), whether and how cargo recruitment affects the initiation and maturation of CCPs is not fully understood. Previous studies have addressed these issues by analyzing the global effects of receptor overexpression on CME or CCP dynamics. Here, we exploit a refined approach using expression of a biotinylated transferrin receptor (bTfnR) and controlling its local clustering using mono- or multivalent streptavidin. We show that local clustering of bTfnR increased CCP initiation. By tracking cargo loading in individual CCPs, we found that bTfnR clustering preceded clathrin assembly and confirmed that bTfnR-containing CCPs mature more efficiently than bTfnR-free CCPs. Although neither the clustering nor the related changes in cargo loading altered the rate of CCP maturation, bTfnR-containing CCPs exhibited significantly longer lifetimes than other CCPs within the same cell. Together these results demonstrate that cargo composition is a key source of the differential dynamics of CCPs.

subject areas

  • Adaptor Protein Complex 2
  • Adaptor Protein Complex sigma Subunits
  • Animals
  • Biotin
  • Biotinylation
  • Carbon-Nitrogen Ligases
  • Cell Line
  • Cell Membrane
  • Cercopithecus aethiops
  • Clathrin Light Chains
  • Coated Pits, Cell-Membrane
  • Endocytosis
  • Epithelial Cells
  • Escherichia coli Proteins
  • Humans
  • Kinetics
  • Protein Binding
  • Rats
  • Receptor Aggregation
  • Receptors, Transferrin
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Streptavidin
  • Transduction, Genetic
scroll to property group menus

Identity

PubMed Central ID

  • PMC3010081

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.201008117

PubMed ID

  • 21187331
scroll to property group menus

Additional Document Info

start page

  • 1381

end page

  • 1393

volume

  • 191

issue

  • 7

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support