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Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells

Academic Article
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Overview

related to degree

  • Wu, Douglass, Ph.D. in Chemistry, Scripps Research 2001 - 2006

authors

  • Wu, Douglass
  • Xing, G. W.
  • Poles, M. A.
  • Horowitz, A.
  • Kinjo, Y.
  • Sullivan, B.
  • Bodmer-Narkevitch, V.
  • Plettenburg, O.
  • Kronenberg, M.
  • Tsuji, M.
  • Ho, D. D.
  • Wong, Chi-Huey

publication date

  • 2005

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist alpha-galactosyl ceramide (alpha-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, alpha-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to alpha-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-gamma secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with alpha-GalCer. Because alpha-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.

subject areas

  • Animals
  • Antigens, Bacterial
  • Antigens, CD1
  • Antigens, CD1d
  • Bacteria
  • Borrelia burgdorferi (UMLS)
  • Cell Line
  • Ceramides
  • Glycolipids
  • Glycosphingolipids
  • Humans
  • Killer Cells, Natural
  • Mice
  • Models, Molecular
  • Molecular Conformation
  • Sphingomonas
  • Sphingosine
  • T-Lymphocytes
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Identity

PubMed Central ID

  • PMC547854

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0408696102

PubMed ID

  • 15665086
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Additional Document Info

start page

  • 1351

end page

  • 1356

volume

  • 102

issue

  • 5

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