Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Francesconi, Valter
  • Cammalleri, M.
  • Sanna, Pietro

publication date

  • 2004

journal

  • Brain Research  Journal

abstract

  • Selective antagonists of the metabotropic receptors 1 (mGluR1), +/-2-methyl-4-carboxyphenylglycine (LY367385), and mGluR5, 2-methyl-6-(phenylethynyl)-pyridine (MPEP), were used to investigate the role of group I metabotropic receptors in late-phase long-term potentiation (L-LTP) at Schaffer collateral/commissural fiber-CA1 synapses in rat hippocampal slices. L-LTP was induced with three trains of tetanization of 1 s duration at 100 Hz separated by 10-min intervals. Neither LY367385 nor MPEP affected basal synaptic responses at the doses used (200 and 10 microM, respectively) and only the mGluR5 inhibitor MPEP blocked L-LTP. However, in agreement with previous mouse mutant studies, we found that both LY367385 and MPEP inhibited the induction of an LTP obtained with a single train of tetanization of 1 s duration at 100 Hz. MPEP's ability to disrupt L-LTP was not due to an effect on NMDA responses since it did not affect pharmacologically isolated N-methyl-D-aspartate (NMDA) excitatory postsynaptic potentials (EPSPs). However, MPEP prevented the increased phosphorylation in dendrites of p70 S6 kinase (p70(S6K)) at Thr3889, a major regulator of translation required for the induction of protein synthesis-dependent forms of LTP.

subject areas

  • Animals
  • Animals, Newborn
  • Benzoates
  • Dendrites
  • Dose-Response Relationship, Drug
  • Dose-Response Relationship, Radiation
  • Electric Stimulation
  • Excitatory Amino Acid Antagonists
  • Excitatory Postsynaptic Potentials
  • Glycine
  • Hippocampus
  • Immunohistochemistry
  • In Vitro Techniques
  • Long-Term Potentiation
  • Neurons
  • Phosphorylation
  • Pyridines
  • Rats
  • Rats, Wistar
  • Receptor, Metabotropic Glutamate 5
  • Receptors, Metabotropic Glutamate
  • Ribosomal Protein S6 Kinases, 70-kDa
  • Threonine
  • Time Factors
scroll to property group menus

Research

keywords

  • excitatory postsynaptic potential
  • memory
  • metabotropic glutamate receptor
  • synaptic plasticity
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0006-8993

Digital Object Identifier (DOI)

  • 10.1016/j.brainres.2004.06.060

PubMed ID

  • 15353208
scroll to property group menus

Additional Document Info

start page

  • 12

end page

  • 18

volume

  • 1022

issue

  • 1-2

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support