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Identification of a novel protein kinase A anchoring protein that binds both type l and type ll regulatory subunits

Academic Article
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Overview

authors

  • Huang, L. J. S.
  • Durick, K.
  • Weiner, J. A.
  • Chun, Jerold
  • Taylor, S. S.

publication date

  • March 1997

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Compartmentalization of cAMP-dependent protein kinase is achieved in part by interaction with A-kinase anchoring proteins (AKAPs). All of the anchoring proteins identified previously target the kinase by tethering the type II regulatory subunit. Here we report the cloning and characterization of a novel anchoring protein, D-AKAP1, that interacts with the N terminus of both type I and type II regulatory subunits. A novel cDNA encoding a 125-amino acid fragment of D-AKAP1 was isolated from a two-hybrid screen and shown to interact specifically with the type I regulatory subunit. Although a single message of 3.8 kilobase pairs was detected for D-AKAP1 in all embryonic stages and in most adult tissues, cDNA cloning revealed the possibility of at least four splice variants. All four isoforms contain a core of 526 amino acids, which includes the R binding fragment, and may be expressed in a tissue-specific manner. This core sequence was homologous to S-AKAP84, including a mitochondrial signal sequence near the amino terminus (Lin, R. Y., Moss, S. B., and Rubin, C. S. (1995) J. Biol. Chem. 270, 27804-27811). D-AKAP1 and the type I regulatory subunit appeared to have overlapping expression patterns in muscle and olfactory epithelium by in situ hybridization. These results raise a novel possibility that the type I regulatory subunit may be anchored via anchoring proteins.

subject areas

  • A Kinase Anchor Proteins
  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • Carrier Proteins
  • Cloning, Molecular
  • Cyclic AMP-Dependent Protein Kinase Type II
  • Cyclic AMP-Dependent Protein Kinases
  • DNA, Complementary
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger
  • Sequence Homology, Amino Acid
  • Substrate Specificity
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 9065479
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Additional Document Info

start page

  • 8057

end page

  • 8064

volume

  • 272

issue

  • 12

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