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Mutational dissociation of the positive and negative regulatory properties of the spo0a sporulation transcription factor of bacillus-subtilis

Academic Article
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Overview

authors

  • Perego, Marta
  • Wu, J. J.
  • Spiegelman, G. B.
  • Hoch, James

publication date

  • April 1991

journal

  • Gene  Journal

abstract

  • The Spo0A regulatory protein controls the onset of stationary phase and sporulation by controlling transcription in both a negative and a positive manner depending on the promoter affected. Missense mutations, e.g., spo0A9V, which result in alterations in the eleventh amino acid preceding the C terminus of the Spo0A protein, give rise to a protein active as a negative regulator of the abrB gene but unable to activate transcription of the spoIIA gene. Second-site suppressors of spo0A9V occurred within the spo0A gene at codons 162 and 174. These suppressors did not suppress a spo0F mutation, indicating that the suppressed protein still requires phosphorylation for activity. The results suggest that the C terminus of Spo0A interacts with the transcription complex to activate transcription.

subject areas

  • Amino Acid Sequence
  • Bacillus subtilis
  • Bacterial Proteins
  • Cloning, Molecular
  • Genes, Suppressor
  • Genotype
  • Molecular Sequence Data
  • Mutagenesis
  • Operon
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Spores, Bacterial
  • Suppression, Genetic
  • Transcription Factors
  • Transcription, Genetic
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Research

keywords

  • ABRB EXPRESSION
  • BETA-GALACTOSIDASE FUSIONS
  • INTERGENIC SUPPRESSORS
  • OA BOX
  • PHOSPHORELAY
  • RECOMBINANT DNA
  • SPOIIA GENE EXPRESSION
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Identity

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(91)90368-l

PubMed ID

  • 1905258
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Additional Document Info

start page

  • 207

end page

  • 212

volume

  • 100

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