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Molecular cloning of protein phosphatase inhibitor-1 and its expression in rat and rabbit tissues

Academic Article
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Overview

authors

  • Elbrecht, A.
  • Direnzo, J.
  • Smith, Roy
  • Shenolikar, S.

publication date

  • August 1990

journal

  • Journal of Biological Chemistry  Journal

abstract

  • A cDNA encoding the complete amino acid sequence of rat protein phosphatase inhibitor-1 was obtained by screening a skeletal muscle library. The coding region represents a 171-residue polypeptide which demonstrated 80% overall identity with the primary sequence of rabbit inhibitor-1. Sequence homology between the rat and rabbit proteins was particularly striking (98% identity) in the NH2-terminal 61 amino acids, which encompass the threonine phosphorylated by cyclic AMP-dependent protein kinase. This domain possesses full inhibitor activity against type-1 protein phosphatases. In contrast, a domain of similar size at the COOH terminus showed only 57% conservation of primary structure between the two proteins. This reflects a remarkable difference in evolutionary pressures experienced by these domains and may emphasize a lesser role for the COOH-terminal region in inhibitor-1 function. Northern hybridization analysis of RNA from rat and rabbit tissues indicated the presence of two mRNAs, a major 0.7-kilobase and a minor 1.8-kilobase mRNA. The highest expression of inhibitor-1 mRNA was noted in skeletal muscle from both species. Analysis of mRNA levels illustrates potential post-transcriptional mechanisms controlling inhibitor-1 expression in some mammalian tissues.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carrier Proteins
  • Cloning, Molecular
  • Enzyme Inhibitors
  • Gene Expression
  • Gene Library
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Muscles
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • Proteins
  • RNA
  • Rats
  • Rats, Inbred Strains
  • Sequence Homology, Nucleic Acid
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 1696252
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Additional Document Info

start page

  • 13415

end page

  • 13418

volume

  • 265

issue

  • 23

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