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pFab-CMV, a single vector system for the rapid conversion of recombinant Fabs into whole IgG1 antibodies

Academic Article
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Overview

authors

  • Sanna, Pietro
  • Samson, M. E.
  • Moon, J. S.
  • Rozenshteyn, R.
  • De Logu, A.
  • Williamson, R. A.
  • Burton, Dennis

publication date

  • 1999

journal

  • Immunotechnology  Journal

abstract

  • We have constructed a single vector system for the rapid conversion of recombinant Fabs into whole IgG1 antibodies and their expression in eukaryotic cells. This vector, named pFab-CMV, utilizes the same unique cloning sites present on the pComb3 phagemid thus allowing for the direct subcloning of light chains and heavy chain Fd regions. pFab-CMV also allows for the expression of recombinant Fabs in eukaryotic cells by removal of a cassette containing part of the hinge, CH2 and CH3 sequences. Stable cell lines are rapidly obtained with pFab-CMV by NEO selection without the need for co-transfection of heavy and light chain expressing vectors.

subject areas

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Bacteriophages
  • CHO Cells
  • Cricetinae
  • Cytomegalovirus
  • Genetic Vectors
  • Humans
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G
  • Molecular Sequence Data
  • Peptide Library
  • Protein Engineering
  • Recombinant Proteins
  • Transfection
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Research

keywords

  • antibody engineering
  • eukaryotic cells
  • expression vector
  • human monoclonal antibodies
  • immunoglobulin expression
  • phage antibody libraries
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Identity

International Standard Serial Number (ISSN)

  • 1380-2933

Digital Object Identifier (DOI)

  • 10.1016/s1380-2933(98)00022-0

PubMed ID

  • 10231088
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Additional Document Info

start page

  • 185

end page

  • 188

volume

  • 4

issue

  • 3-4

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