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Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins

Academic Article
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Overview

related to degree

  • Hu, Tianhua, Ph.D. in Biology, Scripps Research 1991 - 1997

authors

  • Hu, Tianhua
  • Guan, T. L.
  • Gerace, Larry

publication date

  • August 1996

journal

  • Journal of Cell Biology  Journal

abstract

  • Macromolecular trafficking across the nuclear envelope involves interactions between cytosolic transport factors and nuclear pore complex proteins. The p62 complex, an assembly of 62, 58, 54, and 45-kD O-linked glycoproteins-localized near the central gated channel of the nuclear pore complex, has been directly implicated in nuclear protein import. The cDNA cloning of rat p62 was reported previously. We have now carried out cDNA cloning of rat p58, p54, and p45. We found that p58 contains regions with FG (Phe, Gly) and PA (Pro, Ala) repeats at both its NH2 and COOH termini separated by a predicted alpha-helical coiled-coil region, while p54 has an NH2-terminal FG and PA repeat region and a COOH-terminal predicted coiled-coil region. p45 and p58 appear to be generated by alternative splicing, with p45 containing the NH2-terminal FG repeat region and the coiled-coil region of p58. Using immunogold electron microscopy, we found that p58/p45 and p54 are localized on both sides of the nuclear pore complex, like p62. Previous studies have shown that immobilized recombinant p62 can bind the cytosolic nuclear import factor NTF2 and thereby deplete transport activity from cytosol. We have now found that immobilized recombinant p58 and p54 also can deplete nuclear transport activity from cytosol, and that p62, p58, and p54 bind directly to the cytosolic nuclear import factors p97 and NTF2. At least in the case of p58, this involves FG repeat regions. Moreover, p58 can bind to a complex containing transport ligand, the nuclear localization sequence receptor (Srp1 alpha) and p97. These data support a model in which the p62 complex binds to a multicomponent particle consisting of transport ligand and cytosolic factors to achieve accumulation of ligand near the central gated channel of the nuclear pore complex.

subject areas

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Transport
  • Carrier Proteins
  • Cloning, Molecular
  • Cytosol
  • DNA, Complementary
  • HeLa Cells
  • Humans
  • Liver
  • Membrane Glycoproteins
  • Membrane Proteins
  • Molecular Sequence Data
  • Nuclear Envelope
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • Nucleocytoplasmic Transport Proteins
  • Peptide Fragments
  • Protein Binding
  • Protein Structure, Secondary
  • Rats
  • Recombinant Fusion Proteins
  • alpha Karyopherins
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Identity

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.134.3.589

PubMed ID

  • 8707840
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Additional Document Info

start page

  • 589

end page

  • 601

volume

  • 134

issue

  • 3

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