Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Generation and structural analysis of reactive empty particles derived from an icosahedral virus

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Ochoa, W. F.
  • Chatterji, A.
  • Lin, T. W.
  • Johnson Jr., John

publication date

  • 2006

journal

  • Chemistry & Biology  Journal

abstract

  • Chemical and genetic modifications on the surface of viral protein cages confer unique properties to the virus particles with potential nano and biotechnological applications. The enclosed space in the interior of the virus particles further increases its versatility as a nanomaterial. In this paper, we report a simple method to generate a high yield of stable cowpea mosaic virus (CPMV) empty capsids from their native nucleoprotein counterparts by removing the encapsidated viral genome without compromising the integrity of the protein coat. Biochemical and structural comparison of artificially generated empty particles did not reveal any distinguishable differences from CPMV particles containing viral RNA. Preliminary results on the use of artificially produced empty CPMV capsids as a carrier capsule are described.

subject areas

  • Cryoelectron Microscopy
  • Crystallography, X-Ray
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Dyes
  • Hydrolysis
  • Iridoviridae
  • RNA, Viral
  • Virion
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1074-5521

Digital Object Identifier (DOI)

  • 10.1016/j.chembiol.2006.05.014

PubMed ID

  • 16873025
scroll to property group menus

Additional Document Info

start page

  • 771

end page

  • 778

volume

  • 13

issue

  • 7

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support