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Metabolic stress reversibly activates the Drosophila light-sensitive channels TRP and TRPL in vivo

Academic Article
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Overview

authors

  • Agam, K.
  • von Campenhausen, M.
  • Levy, S.
  • Ben-Ami, H. C.
  • Cook, Boaz
  • Kirschfeld, K.
  • Minke, B.

publication date

  • August 2000

journal

  • Journal of Neuroscience  Journal

abstract

  • Drosophila transient receptor potential (TRP) is a prototypical member of a novel family of channel proteins underlying phosphoinositide-mediated Ca(2+) entry. Although the initial stages of this signaling cascade are well known, downstream events leading to the opening of the TRP channels are still obscure. In the present study we applied patch-clamp whole-cell recordings and measurements of Ca(2+) concentration by ion-selective microelectrodes in eyes of normal and mutant Drosophila to isolate the TRP and TRP-like (TRPL)-dependent currents. We report that anoxia rapidly and reversibly depolarizes the photoreceptors and induces Ca(2+) influx into these cells in the dark. We further show that openings of the light-sensitive channels, which mediate these effects, can be obtained by mitochondrial uncouplers or by depletion of ATP in photoreceptor cells, whereas the effects of illumination and all forms of metabolic stress were additive. Effects similar to those found in wild-type flies were also found in mutants with strong defects in rhodopsin, Gq-protein, or phospholipase C, thus indicating that the metabolic stress operates at a late stage of the phototransduction cascade. Genetic elimination of both TRP and TRPL channels prevented the effects of anoxia, mitochondrial uncouplers, and depletion of ATP, thus demonstrating that the TRP and TRPL channels are specific targets of metabolic stress. These results shed new light on the properties of the TRP and TRPL channels by showing that a constitutive ATP-dependent process is required to keep these channels closed in the dark, a requirement that would make them sensitive to metabolic stress.

subject areas

  • 2,4-Dinitrophenol
  • Adenosine Triphosphate
  • Animals
  • Anoxia
  • Calcium Channels
  • Calmodulin-Binding Proteins
  • Dark Adaptation
  • Drosophila Proteins
  • Drosophila melanogaster
  • Insect Proteins
  • Membrane Potentials
  • Membrane Proteins
  • Microelectrodes
  • Mitochondria
  • Mutagenesis
  • NAD
  • Oxidative Stress
  • Patch-Clamp Techniques
  • Photic Stimulation
  • Photoreceptor Cells, Invertebrate
  • Transient Receptor Potential Channels
  • Uncoupling Agents
  • Vision, Ocular
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Research

keywords

  • Drosophila mutants
  • TRP and TRPL channels
  • anoxia
  • ion-selective microelectrodes
  • metabolic stress
  • mitochondrial uncouplers
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Identity

International Standard Serial Number (ISSN)

  • 0270-6474

PubMed ID

  • 10908615
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Additional Document Info

start page

  • 5748

end page

  • 5755

volume

  • 20

issue

  • 15

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