Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Immunochemical studies of the muscarinic acetylcholine-receptor

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Andre, C.
  • Marullo, S.
  • Guillet, J. G.
  • Convents, A.
  • Lauwereys, M.
  • Kaveri, S.
  • Hoebeke, J.
  • Strosberg, Donny

publication date

  • 1987

journal

  • Journal of Receptor Research  Journal

abstract

  • Muscarinic receptors have been purified from calf forebrain plasma cell membranes by affinity chromatography on a dexetimide-agarose gel. SDS-PAGE analysis showed a single 70 kDa band. Monoclonal antibodies have been prepared against these affinity purified 70 kDa protein(s). One antibody, M-35, immunoprecipitated up to 80% of digitonin-solubilized muscarinic receptors. M-35 had agonist-like effects on guinea-pig myometrium: it increased the intracellular cyclic GMP content, decreased prostaglandin-induced cyclic AMP accumulation and caused muscle contractions. The two first effects were inhibited by atropine. M-35 was used to visualize muscarinic receptors at the surface of human fibroblastic cells. In the particular cell line used, the receptors have a low affinity for pirenzepine, were negatively coupled to adenylate cyclase and mediated increase in the phosphatidyl-inositol breakdown.

subject areas

  • Acetylcholine
  • Adenylyl Cyclases
  • Animals
  • Antibodies, Monoclonal
  • Atropine
  • Brain Chemistry
  • Cattle
  • Cell Line
  • Chromatography, Affinity
  • Cyclic GMP
  • Female
  • Fibroblasts
  • Guinea Pigs
  • Humans
  • Pirenzepine
  • Receptors, Muscarinic
  • Uterine Contraction
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0197-5110

Digital Object Identifier (DOI)

  • 10.3109/10799898709054981

PubMed ID

  • 3040987
scroll to property group menus

Additional Document Info

start page

  • 89

end page

  • 103

volume

  • 7

issue

  • 1-4

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support