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TRAPP, a highly conserved novel complex on the cis-Golgi that mediates vesicle docking and fusion

Academic Article
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Overview

authors

  • Sacher, M.
  • Jiang, Y.
  • Barrowman, J.
  • Scarpa, A.
  • Burston, J.
  • Zhang, L.
  • Schieltz, D.
  • Yates III, John
  • Abeliovich, H.
  • Ferro-Novick, S.

publication date

  • May 1998

journal

  • EMBO Journal  Journal

abstract

  • We previously identified BET3 by its genetic interactions with BET1, a gene whose SNARE-like product acts in endoplasmic reticulum (ER)-to-Golgi transport. To gain insight into the function of Bet3p, we added three c-myc tags to its C-terminus and immunopurified this protein from a clarified detergent extract. Here we report that Bet3p is a member of a large complex ( approximately 800 kDa) that we call TRAPP (transport protein particle). We propose that TRAPP plays a key role in the targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment. The localization of Bet3p to the cis-Golgi complex, as well as biochemical studies showing that Bet3p functions on this compartment, support this hypothesis. TRAPP contains at least nine other constituents, five of which have been identified and shown to be highly conserved novel proteins.

subject areas

  • Amino Acid Sequence
  • Conserved Sequence
  • Endoplasmic Reticulum
  • Epitopes
  • Fungal Proteins
  • Golgi Apparatus
  • Green Fluorescent Proteins
  • Intracellular Membranes
  • Luminescent Proteins
  • Macromolecular Substances
  • Membrane Fusion
  • Membrane Proteins
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Peptide Fragments
  • Proto-Oncogene Proteins c-myc
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Vesicular Transport Proteins
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Research

keywords

  • membrane traffic
  • novel complex
  • vesicle targeting and fusion
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Identity

PubMed Central ID

  • PMC1170591

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1093/emboj/17.9.2494

PubMed ID

  • 9564032
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Additional Document Info

start page

  • 2494

end page

  • 2503

volume

  • 17

issue

  • 9

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