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Antibodies against synthetic peptides react with the 2nd epstein-barr virus-associated nuclear antigen

Academic Article
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Overview

authors

  • Dillner, J.
  • Kallin, B.
  • Klein, G.
  • Jornvall, H.
  • Alexander, H.
  • Lerner, Richard

publication date

  • 1985

journal

  • EMBO Journal  Journal

abstract

  • Five peptides were synthesized on the basis of amino acid sequences predicted from the transformation-associated BamHI WYH region of the genome of the Epstein-Barr virus (EBV). Antisera to two peptides deduced from a 1.6-kb open reading frame in the BamHI H fragment identified an 87 000-dalton nuclear polypeptide that was present in EBV-carrying cell lines that expressed the second EBV-determined nuclear antigen (EBNA-2). This polypeptide was not detected in cell lines that carried EBV variants with a deleted BamHI WYH region or in EBV-negative cell lines. Three peptides deduced from the 1.6-kb open reading frame reacted with human EBNA-positive sera, but not with EBNA-negative sera. Following affinity purification with the peptides, two of the corresponding human antibodies also reacted with the 87 000-dalton polypeptide.

subject areas

  • Amino Acid Sequence
  • Antibodies
  • Antigen-Antibody Complex
  • Antigens, Viral
  • Cell Line
  • DNA Restriction Enzymes
  • Enzyme-Linked Immunosorbent Assay
  • Herpesvirus 4, Human
  • Humans
  • Peptides
  • Structure-Activity Relationship
  • Viral Matrix Proteins
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Identity

PubMed Central ID

  • PMC554422

International Standard Serial Number (ISSN)

  • 0261-4189

PubMed ID

  • 2992945
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Additional Document Info

start page

  • 1813

end page

  • 1818

volume

  • 4

issue

  • 7

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