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Ventricular zone gene-1 (vzg-1) encodes a lysophosphatidic acid receptor expressed in neurogenic regions of the developing cerebral cortex

Academic Article
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Overview

authors

  • Hecht, J. H.
  • Weiner, J. A.
  • Post, S. R.
  • Chun, Jerold

publication date

  • November 1996

journal

  • Journal of Cell Biology  Journal

abstract

  • Neocortical neuroblast cell lines were used to clone G-protein-coupled receptor (GPCR) genes to study signaling mechanisms regulating cortical neurogenesis. One putative GPCR gene displayed an in situ expression pattern enriched in cortical neurogenic regions and was therefore named ventricular zone gene-1 (vzg-1). The vzg-1 cDNA hybridized to a 3.8-kb mRNA transcript and encoded a protein with a predicted molecular mass of 41-42 kD, confirmed by Western blot analysis. To assess its function, vzg-1 was overexpressed in a cell line from which it was cloned, inducing serum-dependent "cell rounding." Lysophosphatidic acid (LPA), a bioactive lipid present in high concentrations in serum, reproduced the effect seen with serum alone. Morphological responses to other related phospholipids or to thrombin, another agent that induces cell rounding through a GPCR, were not observed in vzg-1 overexpressing cells. Vzg-1 overexpression decreased the EC50 of both cell rounding and Gi activation in response to LPA. Pertussis toxin treatment inhibited vzg-1-dependent LPA-mediated Gi activation, but had no effect on cell rounding. Membrane binding studies indicated that vzg-1 overexpression increased specific LPA binding. These analyses identify the vzg-1 gene product as a receptor for LPA, suggesting the operation of LPA signaling mechanisms in cortical neurogenesis. Vzg-1 therefore provides a link between extracellular LPA and the activation of LPA-mediated signaling pathways through a single receptor and will allow new investigations into LPA signaling both in neural and nonneural systems.

subject areas

  • Age Factors
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Size
  • Cerebral Cortex
  • Dose-Response Relationship, Drug
  • Embryo, Mammalian
  • Female
  • GTP-Binding Proteins
  • Gene Expression Regulation, Developmental
  • Lysophospholipids
  • Membrane Proteins
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neurons
  • Pregnancy
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Receptors, Lysophosphatidic Acid
  • Signal Transduction
  • Transfection
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Identity

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.135.4.1071

PubMed ID

  • 8922387
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Additional Document Info

start page

  • 1071

end page

  • 1083

volume

  • 135

issue

  • 4

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