Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

CD4 is expressed on murine pluripotent hematopoietic stem cells

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Wineman, J. P.
  • Gilmore, G. L.
  • Gritzmacher, C.
  • Torbett, Bruce
  • Mullersieburg, C. E.

publication date

  • October 1992

journal

  • Blood  Journal

abstract

  • We show here for the first time that pluripotent hematopoietic stem cells express the CD4 antigen. CD4+ cells isolated from mouse marrow repopulated all hematopoietic lineages in both the long-term repopulation assay and the competitive repopulation assay. This finding indicates that the CD4+ population contains primitive stem cells with extensive repopulation capacity. Interestingly, the CD4- population had significant life-sparing activity, even though this population was depleted of long-term repopulating stem cells when compared with CD4+ cells. The majority of the cells that respond to the stroma in Whitlock-Witte cultures with B-cell differentiation were recovered in the CD4- population. Thus, this bone marrow (BM)-derived B-cell precursor lacks CD4, which is in contrast to myeloid precursors and thymus-derived lymphoid precursors that reportedly express CD4. We show further that the CD4 molecule expressed on BM cells is similar in molecular weight and epitope makeup to the CD4 antigen found on thymocytes. Detection of CD4 on BM cells is dependent on using high concentrations of antibodies. Thus, it is not surprising that expression of CD4 on pluripotent stem cells has been missed previously. Taken together, our data suggest that the CD4 molecule may play an important role in lineage definition in early hematopoietic differentiation.

subject areas

  • Animals
  • Antibodies
  • Antigens, CD4
  • Bone Marrow
  • Bone Marrow Cells
  • Cell Separation
  • Cells, Cultured
  • Fluorescent Antibody Technique
  • Hematopoietic Stem Cells
  • Lymphocyte Depletion
  • Mice
  • Mice, Inbred C57BL
  • T-Lymphocytes
  • Thymus Gland
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0006-4971

PubMed ID

  • 1391941
scroll to property group menus

Additional Document Info

start page

  • 1717

end page

  • 1724

volume

  • 80

issue

  • 7

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support