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Conformation of cd7 -metallothionein-2 from rat-liver in aqueous-solution determined by nuclear magnetic-resonance spectroscopy

Academic Article
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Overview

authors

  • Schultze, P.
  • Worgotter, E.
  • Braun, W.
  • Wagner, G.
  • Vasak, M.
  • Kagi, J. H. R.
  • Wuthrich, Kurt

publication date

  • September 1988

journal

  • Journal of Molecular Biology  Journal

abstract

  • The three-dimensional structure of [Cd7]-metallothionein-2 from rat liver was determined in aqueous solution, using nuclear magnetic resonance spectrometry and distance geometry calculations. The experimental data provided proton-proton distance constraints from measurements of nuclear Overhauser effects, constraints on the geometry of the metal-cysteine clusters determined by heteronuclear correlation spectroscopy, and dihedral angle constraints derived from both coupling constants and nuclear Overhauser effects. The structure calculations were performed with the program DISMAN. As in previous studies with rabbit liver metallothionein-2a, the structure calculations were performed separately for the alpha and beta-domains containing the 4 and 3-metal clusters, respectively, since no interdomain constraints were found. For both domains, the global polypeptide fold, the location of polypeptide secondary structure elements, the architecture of the metal-sulfur cluster and the local chirality of the metal co-ordination are very similar to the solution structure of rabbit metallothionein-2a, but show considerable difference relative to the crystal structure of rat metallothionein-2.

subject areas

  • Animals
  • Cadmium
  • Cysteine
  • Liver
  • Magnetic Resonance Spectroscopy
  • Mathematical Computing
  • Metallothionein
  • Models, Molecular
  • Protein Conformation
  • Rabbits
  • Rats
  • Solutions
  • X-Ray Diffraction
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Identity

International Standard Serial Number (ISSN)

  • 0022-2836

Digital Object Identifier (DOI)

  • 10.1016/0022-2836(88)90106-4

PubMed ID

  • 3184190
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Additional Document Info

start page

  • 251

end page

  • 268

volume

  • 203

issue

  • 1

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