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Chronic exposure to pertussis toxin alters muscarinic receptor-mediated regulation of cyclic AMP metabolism in neuroblastoma x glioma NG108-15 hybrid cells

Academic Article
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Overview

authors

  • Westlinddanielsson, A.
  • Gillenius, P.
  • Askelof, P.
  • Bartfai, Tamas

publication date

  • July 1988

journal

  • Journal of Neurochemistry  Journal

abstract

  • Chronic pertussis toxin treatment (5 days) of NG108-15 neuroblastoma X glioma hybrid cells had no significant effect on basal cyclic AMP levels whereas it effectively blocked the inhibitory action of acute (10 min) exposure of carbachol (10(-4)M) on intracellular cyclic AMP accumulation, stimulated by prostaglandin E1. This action of pertussis toxin was found to be long lasting: exposure of the cells to pertussis toxin (100 ng/ml) for only 24 h followed by a 5-day withdrawal period still was shown effective on day 7 in abolishing the inhibitory action of carbachol on prostaglandin E1-stimulated cyclic AMP production. Chronic exposure (5 days) of NG108-15 cells to carbachol (10(-5)M) causes an increase in basal cyclic AMP levels by 98%, and a desensitization of the muscarinic inhibition of cyclic AMP accumulation, assessed after a 24-h withdrawal period. When carbachol treatment is carried out in the presence of pertussis toxin (100 ng/ml) both of these effects of carbachol are abolished.

subject areas

  • 1-Methyl-3-isobutylxanthine
  • Alprostadil
  • Atropine
  • Carbachol
  • Cyclic AMP
  • Glioma
  • Hybrid Cells
  • Neuroblastoma
  • Pertussis Toxin
  • Receptors, Muscarinic
  • Tumor Cells, Cultured
  • Virulence Factors, Bordetella
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Identity

International Standard Serial Number (ISSN)

  • 0022-3042

Digital Object Identifier (DOI)

  • 10.1111/j.1471-4159.1988.tb04832.x

PubMed ID

  • 2454296
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Additional Document Info

start page

  • 38

end page

  • 44

volume

  • 51

issue

  • 1

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