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A role for Hsp90 in cell cycle control: Wee1 tyrosine kinase activity requires interaction with Hsp90

Academic Article
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Overview

authors

  • Aligue, R.
  • Akhavanniak, H.
  • Russell, Paul

publication date

  • December 1994

journal

  • EMBO Journal  Journal

abstract

  • Wee1 protein kinase regulates the length of G2 phase by carrying out the inhibitory tyrosyl phosphorylation of Cdc2-cyclin B kinase. Mutations were isolated that suppressed the G2 cell cycle arrest caused by overproduction of Wee1. One class of swo (suppressor of wee1 overproduction) mutation, exemplified by swo1-26, also caused a temperature sensitive lethal phenotype in a wee1+ background. The swo1+ gene encodes a member of the Hsp90 family of stress proteins. Swo1 is essential for viability at all temperatures. Swo1 coimmunoprecipitates with Wee1, showing that the two proteins interact. The swo1-26 mutant undergoes premature mitosis when grown at a semi-permissive temperature. These data strongly indicate that formation of active Wee1 tyrosine kinase requires interaction with Swo1, perhaps in a manner analogous to the previously demonstrated interaction between Hsp90 and v-src tyrosine kinase. These observations demonstrate a unexpected role for Hsp90 in cell cycle control.

subject areas

  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle
  • Cell Cycle Proteins
  • Enzyme Stability
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Genes, Lethal
  • HSP90 Heat-Shock Proteins
  • Hot Temperature
  • Mitosis
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins
  • Protein-Tyrosine Kinases
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Sequence Homology, Amino Acid
  • Suppression, Genetic
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Research

keywords

  • CELL CYCLE
  • HEAT SHOCK PROTEIN
  • HSP90
  • MITOSIS
  • WEE1
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Identity

PubMed Central ID

  • PMC395589

International Standard Serial Number (ISSN)

  • 0261-4189

PubMed ID

  • 7813446
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Additional Document Info

start page

  • 6099

end page

  • 6106

volume

  • 13

issue

  • 24

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