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Virus-lymphocyte interactions .2. Expression of viral sequences during the course of persistent lymphocytic choriomeningitis virus-infection and their localization to the lymphocyte-l3t4 subset

Academic Article
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Overview

authors

  • Tishon, A.
  • Southern, P. J.
  • Oldstone, Michael

publication date

  • February 1988

journal

  • Journal of Immunology  Journal

abstract

  • Viruses that cause in vivo persistent infections need to selectively compromise the host's immunologic surveillance machinery in order to survive. To understand the molecular basis of how this is accomplished we have analyzed persistent virus infection by lymphocytic choriomeningitis in its normal host, the mouse. Earlier we noted by infectious center analysis that five in 10(4) lymphocytes carried by persistently infected mice contained infectious materials throughout the course of infection. A previous publication extended these results, in BALB mice by showing that the L3T4+ lymphocyte subset in lymph nodes and spleens was predominantly involved. Using cDNA labeled probes to the viral genome and in situ hybridization we report that 1 to 2% of circulating lymphocytes from several mouse strains contain viral RNA sequences for the three viral structural genes. By FACS analysis, the Thy-1.2+, L3T4+ subset primarily harbors virus while viral sequences are usually not detected in the Lyt-2+ subset as early as 6 days after initiating infection in newborns and throughout the course of the persistence. These findings suggest that incomplete, presumably defective, virus is generated in a subset of Th lymphocytes during persistent infection and that during this time infection of cytotoxic T cell subsets is minimal.

subject areas

  • Animals
  • Animals, Newborn
  • Antigens, Differentiation, T-Lymphocyte
  • Carrier State
  • Chronic Disease
  • DNA
  • DNA, Viral
  • Defective Viruses
  • Genes, Viral
  • Lymphocytic Choriomeningitis
  • Lymphocytic choriomeningitis virus
  • Mice
  • Mice, Inbred Strains
  • T-Lymphocytes
  • T-Lymphocytes, Helper-Inducer
  • Virus Replication
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 2963865
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Additional Document Info

start page

  • 1280

end page

  • 1284

volume

  • 140

issue

  • 4

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