Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Human p53 and cdc2hs genes combine to inhibit the proliferation of saccharomyces-cerevisiae

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Nigro, J. M.
  • Sikorski, R.
  • Reed, Steven
  • Vogelstein, B.

publication date

  • March 1992

journal

  • Molecular and Cellular Biology  Journal

abstract

  • Human wild-type and mutant p53 genes were expressed under the control of a galactose-inducible promoter in Saccharomyces cerevisiae. The growth rate of the yeast was reduced in cells expressing wild-type p53, whereas cells transformed with mutant p53 genes derived from human tumors were less affected. Coexpression of the normal p53 protein with the human cell cycle-regulated protein kinase CDC2Hs resulted in much more pronounced growth inhibition that for p53 alone. Cells expressing p53 and CDC2Hs were partially arrested in G1, as determined by morphological analysis and flow cytometry. p53 was phosphorylated when expressed in the yeast, but differences in phosphorylation did not explain the growth inhibition attributable to coexpression of p53 and CDC2Hs. These results suggest that wild-type p53 has a growth-inhibitory activity in S. cerevisiae similar to that observed in mammalian cells and suggests that this yeast may provide a useful model for defining the pathways through which p53 acts.

subject areas

  • Base Sequence
  • Blotting, Western
  • CDC2 Protein Kinase
  • Cloning, Molecular
  • DNA
  • Electrophoresis, Gel, Two-Dimensional
  • Flow Cytometry
  • Humans
  • Molecular Sequence Data
  • Peptide Mapping
  • Phenotype
  • Phosphorylation
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae
  • Transformation, Genetic
  • Tumor Suppressor Protein p53
scroll to property group menus

Identity

PubMed Central ID

  • PMC369569

International Standard Serial Number (ISSN)

  • 0270-7306

PubMed ID

  • 1545817
scroll to property group menus

Additional Document Info

start page

  • 1357

end page

  • 1365

volume

  • 12

issue

  • 3

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support