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Synthetic peptide immunoassay distinguishes hiv type-1 and hiv type-2 infections

Academic Article
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Overview

authors

  • Gnann, J. W.
  • McCormick, J. B.
  • Mitchell, S.
  • Nelson, J. A.
  • Oldstone, Michael

publication date

  • September 1987

journal

  • Science  Journal

abstract

  • Efforts to solve the epidemiologic puzzle of AIDS in Africa are complicated by the presence of multiple human retroviruses. Simple serologic tests that unambiguously distinguish among infections by these retroviruses are essential. To that end, a partially conserved immunoreactive epitope was identified in the transmembrane glycoproteins of human immunodeficiency viruses (HIV) types 1 and 2. Synthetic peptides derived from these conserved domains were used in sensitive and specific immunoassays that detect antibodies in sera from patients infected with HIV-1 or HIV-2. By making single amino acid substitutions in the HIV-1 peptide, it was possible to demonstrate HIV-1 strain-specific antibody responses to this epitope. Such custom-designed peptides synthesized from this domain are likely to detect newly discovered HIV types, define infection with specific HIV strains, and allow detection of group-common antibodies.

subject areas

  • Amino Acid Sequence
  • Deltaretrovirus Infections
  • Diagnosis, Differential
  • Enzyme-Linked Immunosorbent Assay
  • Glycoproteins
  • Humans
  • Leukemia, Hairy Cell
  • Peptides
  • Viral Envelope Proteins
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Identity

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.2888192

PubMed ID

  • 2888192
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Additional Document Info

start page

  • 1346

end page

  • 1349

volume

  • 237

issue

  • 4820

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