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Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene

Academic Article
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Overview

authors

  • Davis, Ronald
  • Takayasu, H.
  • Eberwine, M.
  • Myres, J.

publication date

  • May 1989

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • A probe representing the Drosophila dunce+ (dnc+) gene, the structural gene for a cAMP phosphodiesterase (PDEase), detects homologous sequences in many different organisms, including mouse, rat, and human. Genomic and cDNA clones representing a homolog of the Drosophila dnc+ gene were isolated from rat libraries and characterized. This gene has been named ratdnc-1. One cDNA clone defines a large open reading frame of approximately 1.8 kilobases (kb), predicting a protein sequence of 610 amino acids with significant homology to a conserved domain of approximately 275 residues found in most other PDEases. The amino acid identity value to the Drosophila cAMP PDEase within this domain is a striking 75%. Other cDNA clones show blocks of sequence divergence from this cDNA clone close to the predicted N terminus, indicating the potential existence of a family of related enzymes encoded by alternatively spliced messenger RNAs from ratdnc-1. Genomic blotting experiments suggest the existence of at least one other rat gene with homology to ratdnc-1. RNAs homologous to ratdnc-1 are heterogeneous in size between tissues, with heart containing a major transcript of 4.4 kb and brain one of 4.0 kb. The potential identity of the product of the ratdnc-1 gene with known PDEases is discussed.

subject areas

  • 3',5'-Cyclic-AMP Phosphodiesterases
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA
  • Drosophila melanogaster
  • Gene Expression Regulation
  • Memory
  • Molecular Sequence Data
  • Rats
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Tissue Distribution
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Identity

PubMed Central ID

  • PMC287186

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.86.10.3604

PubMed ID

  • 2542942
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Additional Document Info

start page

  • 3604

end page

  • 3608

volume

  • 86

issue

  • 10

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