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The application of mass spectrometry to membrane proteomics

Academic Article
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Overview

authors

  • Wu, Chunlei
  • Yates III, John

publication date

  • March 2003

journal

  • Nature Biotechnology  Journal

abstract

  • Membrane proteins perform some of the most important functions in the cell, including the regulation of cell signaling through surface receptors, cell-cell interactions, and the intracellular compartmentalization of organelles. Recent developments in proteomic strategies have focused on the inclusion of membrane proteins in high-throughput analyses. While slow and steady progress continues to be made in gel-based technologies, significant advances have been reported in non-gel shotgun methods using liquid chromatography coupled to mass spectrometry (LC/MS). These latter strategies facilitate the identification of large numbers of membrane proteins and modifications, and have the potential to provide insights into protein topology and orientation in membranes.

subject areas

  • Amino Acid Sequence
  • Chromatography, Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Mass Spectrometry
  • Membrane Proteins
  • Models, Molecular
  • Molecular Weight
  • Protein Conformation
  • Protein Processing, Post-Translational
  • Proteomics
  • Tissue Distribution
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Identity

International Standard Serial Number (ISSN)

  • 1087-0156

Digital Object Identifier (DOI)

  • 10.1038/nbt0303-262

PubMed ID

  • 12610573
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Additional Document Info

start page

  • 262

end page

  • 267

volume

  • 21

issue

  • 3

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