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Bead injection ELISA for the determination of antibodies implicated in type 1 diabetes mellitus

Academic Article
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Overview

authors

  • Carroll, A. D.
  • Scampavia, Louis
  • Luo, D.
  • Lernmark, A.
  • Ruzicka, J.

publication date

  • 2003

journal

  • Analyst  Journal

abstract

  • This work introduces a novel analytical method for the detection and study of GAD65 autoantibodies, which have been implicated in the onset of type 1 diabetes. There is a clinical need for a rapid and automated assay for GAD65 autoantibodies. Therefore, this method was designed to exploit the advantages of bead injection (BI) analysis for enzyme-linked immunosorbent assays (ELISA). BI ELISA is a microscale technique that uses enzyme labeled secondary antibodies to detect the capture of target antibodies on immobilized antigen in the flow cell of the lab-on-valve (LOV) manifold. A detection limit of 20 ng mL(-1) of GAD65 monoclonal antibody 144 compares favorably with the sensitivity and precision of a standard ELISA currently employed to detect GAD65 autoantibodies. Compared to the standard ELISA protocol, BI ELISA offers a significantly reduced assay time and complete automation of solution handling and detection.

subject areas

  • Autoanalysis
  • Autoantibodies
  • Diabetes Mellitus, Type 1
  • Enzyme-Linked Immunosorbent Assay
  • Glutamate Decarboxylase
  • Humans
  • Isoenzymes
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Identity

International Standard Serial Number (ISSN)

  • 0003-2654

Digital Object Identifier (DOI)

  • 10.1039/b302786d

PubMed ID

  • 14529023
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Additional Document Info

start page

  • 1157

end page

  • 1162

volume

  • 128

issue

  • 9

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