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Chromosomal variation in neurons of the developing and adult mammalian nervous system

Academic Article
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Overview

authors

  • Rehen, S. K.
  • McConnell, M. J.
  • Kaushal, D.
  • Kingsbury, M. A.
  • Yang, A. H.
  • Chun, Jerold

publication date

  • November 2001

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • A basic assumption about the normal nervous system is that its neurons possess identical genomes. Here we present direct evidence for genomic variability, manifested as chromosomal aneuploidy, among developing and mature neurons. Analysis of mouse embryonic cerebral cortical neuroblasts in situ detected lagging chromosomes during mitosis, suggesting the normal generation of aneuploidy in these somatic cells. Spectral karyotype analysis identified approximately 33% of neuroblasts as aneuploid. Most cells lacked one chromosome, whereas others showed hyperploidy, monosomy, and/or trisomy. The prevalence of aneuploidy was reduced by culturing cortical explants in medium containing fibroblast growth factor 2. Interphase fluorescence in situ hybridization on embryonic cortical cells supported the rate of aneuploidy observed by spectral karyotyping and detected aneuploidy in adult neurons. Our results demonstrate that genomes of developing and adult neurons can be different at the level of whole chromosomes.

subject areas

  • Aneuploidy
  • Animals
  • Cerebral Cortex
  • Chromosomes
  • Female
  • Flow Cytometry
  • Genetic Variation
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Interphase
  • Karyotyping
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neurons
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Identity

PubMed Central ID

  • PMC60876

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.231487398

PubMed ID

  • 11698687
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Additional Document Info

start page

  • 13361

end page

  • 13366

volume

  • 98

issue

  • 23

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