Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Cationic drug analysis using matrix-assisted laser-desorption ionization mass-spectrometry - application to influx kinetics, multidrug-resistance, and intracellular chemical-change

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Rideout, D.
  • Bustamante, A.
  • Siuzdak, Gary

publication date

  • November 1993

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Highly sensitive and convenient analysis of intracellular cationic drugs has been achieved by applying matrix-assisted laser desorption/ionization mass spectrometry (MALD-MS). Tetraphenylphosphonium cation was readily identified and quantified (using methyltriphenylphosphonium cation as an internal standard) at subpicomole levels in crude lysate from < 4 x 10(3) FaDu human hypopharyngeal carcinoma cells. A quantitative MALD-MS time course for tetraphenylphosphonium cation accumulation into FaDu cells was comparable to a time course using scintillation counting with tritiated tetraphenylphosphonium. MALD-MS was also capable of demonstrating the reduced accumulation of the cationic drug rhodamine-123 by DoxR MCF7, a multiply drug-resistant human breast adenocarcinoma cell line, relative to the nonresistant parent line MCF7. In addition, MALD-MS was used to follow a chemical reaction inside intact FaDu cells: the formation of a hydrazone (II-51) from benzaldehyde and an acylhydrazide, 5-[tris(4-dimethylaminophenyl)phosphonio]pentanoyl hydrazide (II-25). These results suggest that MALD-MS may provide a rapid and practical alternative to existing methods for the analysis of cationic drugs, toxins, and their metabolites in cells and tissues.

subject areas

  • Adenocarcinoma
  • Benzaldehydes
  • Biological Transport
  • Breast Neoplasms
  • Carcinoma, Squamous Cell
  • Cations
  • Cell Line
  • Doxorubicin
  • Drug Resistance
  • Female
  • Humans
  • Hypopharyngeal Neoplasms
  • Kinetics
  • Lasers
  • Mass Spectrometry
  • Molecular Structure
  • Onium Compounds
  • Organophosphorus Compounds
  • Structure-Activity Relationship
  • Trityl Compounds
  • Tumor Cells, Cultured
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.90.21.10226

PubMed ID

  • 8234281
scroll to property group menus

Additional Document Info

start page

  • 10226

end page

  • 10229

volume

  • 90

issue

  • 21

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support