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Measurement of virus antigens on surfaces of hela-cells persistently infected with wild-type and vaccine strains of measles-virus by radioimmune assay

Academic Article
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Overview

authors

  • Joseph, B. S.
  • Perrin, L. H.
  • Oldstone, Michael

publication date

  • 1976

journal

  • Journal of General Virology  Journal

abstract

  • Persistent states of measles virus infection have been established in HeLa cells by using Edmonston strain virus and two types of measles virus vaccine (M-VAC and Schwarz). The absolute amount of surface viral antigens expressed on these cells infected separately with the three viruses has been assessed by a newly developed method which employs [125I]-labelled Fab fragments of immunoglobulin G (IgG) from immune human sera. This method was used to determine the level of viral antigenic expression on acutely infected HeLa cells harvested at a time when 95 to 100% of cells could be lysed by antiviral antibody and complement. From our data, more than 1 X 10(6) antibody molecules must bind to each cell infected with measles virus before complement dependent lysis can occur in a homologous test system. Persistently infected cells bind 2 to 3 times less antibody than acutely infected cells and correspondingly exhibit less susceptibility to humorally-mediated immune lysis.

subject areas

  • Antigens, Viral
  • Cell Membrane
  • HeLa Cells
  • Measles Vaccine
  • Radioimmunoassay
  • Species Specificity
  • Vaccines, Attenuated
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Identity

International Standard Serial Number (ISSN)

  • 0022-1317

Digital Object Identifier (DOI)

  • 10.1099/0022-1317-30-3-329

PubMed ID

  • 1255135
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Additional Document Info

start page

  • 329

end page

  • 337

volume

  • 30

issue

  • MAR

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