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Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein

Academic Article
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Overview

authors

  • Panicker, L. M.
  • Zhang, J. H.
  • Posokhova, E.
  • Gastinger, M. J.
  • Martemyanov, Kirill
  • Simonds, W. F.

publication date

  • June 2010

journal

  • Journal of Neurochemistry  Journal

abstract

  • The neuronally expressed G beta(5) subunit is the most structurally divergent among heterotrimeric G beta isoforms and unique in its ability to heterodimerize with the R7 subfamily of regulator of G protein signaling (RGS) proteins. The complex between G beta(5) and R7-type RGS proteins targets the cell nucleus by an unknown mechanism. Although the nuclear targeting of the G beta(5)/R7-RGS complex is proposed to involve the binding of R7-binding protein (R7BP), this theory is challenged by the observations that endogenous R7BP is palmitoylated, co-localizes strongly with the plasma membrane, and has never been identified in the cytosol or nucleus of native neurons or untreated cultured cells. We show here mutant RGS7 lacking the N-terminal Disheveled, EGL-10, Pleckstrin homology domain is expressed in transfected cells but, unlike wild-type RGS7, is excluded from the cell nucleus. As the Disheveled, EGL-10, Pleckstrin homology domain is essential for R7BP binding to RGS7, we studied the subcellular localization of G beta(5) in primary neurons and brain from mice deficient in R7BP. The level of endogenous nuclear G beta(5) and RGS7 in neurons and brains from R7BP knockout mice is reduced by 50-70%. These results suggest that R7BP contributes significantly to the nuclear localization of endogenous G beta(5)/R7-RGS complex in brain.

subject areas

  • Animals
  • Brain Chemistry
  • Cell Membrane
  • Cell Nucleus
  • Cells, Cultured
  • Cytoplasm
  • DNA, Complementary
  • Fluorescent Antibody Technique
  • GTP-Binding Protein beta Subunits
  • Genotype
  • Immunoprecipitation
  • Mice
  • Mice, Knockout
  • Microscopy, Confocal
  • Mutation
  • Nuclear Localization Signals
  • PC12 Cells
  • RGS Proteins
  • Rats
  • Transfection
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Research

keywords

  • DEP domain
  • R7 binding protein
  • heterotrimeric G protein
  • palmitoylation
  • regulator of G protein signaling
  • subcellular localization
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Identity

PubMed Central ID

  • PMC2890294

International Standard Serial Number (ISSN)

  • 0022-3042

Digital Object Identifier (DOI)

  • 10.1111/j.1471-4159.2010.06616.x

PubMed ID

  • 20100282
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Additional Document Info

start page

  • 1101

end page

  • 1112

volume

  • 113

issue

  • 5

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