Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli

Academic Article
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publication date

  • August 2002

abstract

  • We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.