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Addition of p-azido-L-phenylaianine to the genetic code of Escherichia coli

Academic Article
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Overview

authors

  • Chin, J. W.
  • Santoro, Stephen
  • Martin, A. B.
  • King, D. S.
  • Wang, L.
  • Schultz, Peter

publication date

  • August 2002

journal

  • Journal of the American Chemical Society  Journal

abstract

  • We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.

subject areas

  • Azides
  • Chloramphenicol O-Acetyltransferase
  • Cross-Linking Reagents
  • DNA, Bacterial
  • Escherichia coli
  • Methanococcus
  • Myoglobin
  • Phenylalanine
  • Spectrometry, Mass, Electrospray Ionization
  • Tyrosine-tRNA Ligase
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Identity

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja027007w

PubMed ID

  • 12148987
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Additional Document Info

start page

  • 9026

end page

  • 9027

volume

  • 124

issue

  • 31

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