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Reactive oxygen species modulate the differentiation of neurons in clonal cortical cultures

Academic Article
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Overview

authors

  • Tsatmali, M.
  • Walcott, E. C.
  • Makarenkova, Helen
  • Crossin, Kathryn

publication date

  • 2006

journal

  • Molecular and Cellular Neuroscience  Journal

abstract

  • Reactive oxygen species (ROS) are important regulators of intracellular signaling. We examined the expression of ROS during rat brain development and explored their role in differentiation using cortical cultures. High levels of ROS were found in newborn neurons. Neurons produced ROS, not connected with cell death, throughout embryogenesis and postnatal stages. By P20, ROS-producing cells were found only in neurogenic regions. Cells with low levels of ROS, isolated from E15 brains by FACS, differentiated into neurons, oligodendrocytes, and astrocytes in clonal cultures. Neurons produced high ROS early in culture and later differentiated into two types: large pyramidal-like neurons that fired no or only a single action potential and smaller neurons that expressed nuclear calretinin and fired repeated action potentials. Antioxidant treatment did not alter neuron number but increased the ratio of small to large neurons. These findings suggest that modulation of ROS levels influences multiple aspects of neuronal differentiation.

subject areas

  • Analysis of Variance
  • Animals
  • Animals, Newborn
  • Cell Differentiation
  • Cells, Cultured
  • Cerebral Cortex
  • Electric Stimulation
  • Embryo, Mammalian
  • Flow Cytometry
  • Gene Expression Regulation, Developmental
  • Immunohistochemistry
  • In Vitro Techniques
  • Membrane Potentials
  • Microscopy, Confocal
  • Neurons
  • Patch-Clamp Techniques
  • Rats
  • Reactive Oxygen Species
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Identity

PubMed Central ID

  • PMC1797198

International Standard Serial Number (ISSN)

  • 1044-7431

Digital Object Identifier (DOI)

  • 10.1016/j.mcn.2006.08.005

PubMed ID

  • 17000118
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Additional Document Info

start page

  • 345

end page

  • 357

volume

  • 33

issue

  • 4

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