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Identification of effector cell protease receptor-1 - a leukocyte-distributed receptor for the serine protease factor xa

Academic Article
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Overview

authors

  • Altieri, D. C.
  • Edgington, Thomas

publication date

  • July 1990

journal

  • Journal of Immunology  Journal

abstract

  • Mitogenesis, cell differentiation and immune-inflammatory responses are regulated by the coordinated assembly of proteases with specific cellular receptors. We have investigated the possibility that immune effector cells may express a high-affinity protease receptor. To address this hypothesis, we have generated mAb to factor V and its activated form Va, a circulating plasma protein that binds the serine protease of the coagulation cascade, factor Xa. Further, by flow microfluorimetry screening, we have isolated a panel of these mAb that recognize a surface molecule expressed on transformed monocytic cells. We now show that these mAb bind to blood monocytes, to CD3- CD16+ CD56+ NK cells, and with considerable heterogeneity, to neutrophils. A small subset of CD3+ cells (5 to 10%) was also identified by these probes and further phenotypically characterized by two-color flow microfluorimetry as predominantly coexpressing CD2, CD4 or CD8, CD57, CD11b, and alpha/beta TCR. This subset of CD3+ cells was expanded in vitro by both lectin- or Ag-specific stimulation. In addition, long term alloreactive stimulation resulted in approximately 8- to 10-fold increased expression of the molecule recognized by these mAb. Functional analyses were performed on a selected T cell clonal derivative of the transformed cell line HuT 78. These cells bound 125I-factor Xa in a specific reaction saturated at 194,000 +/- 26,000 molecules/cell with a Kd approximately 10 to 20 nM and inhibited by the mAb panel described above. These data suggest that immune effector cells express a dynamically regulated protease receptor that is immunologically related to the plasma coagulation protein factor V and its activated form Va. We propose the term effector cell protease receptor-1 to tentatively identify this molecule, and we speculate on its possible involvement in specialized protease-mediated effector functions.

subject areas

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, CD3
  • Antigens, Differentiation, T-Lymphocyte
  • Cell Line
  • Factor V
  • Factor Va
  • Factor Xa
  • Flow Cytometry
  • Humans
  • Immunity, Cellular
  • Inhibitor of Apoptosis Proteins
  • Integrins
  • Killer Cells, Natural
  • Lymphocyte Activation
  • Lymphocytes
  • Molecular Weight
  • Monocytes
  • Neutrophils
  • Precipitin Tests
  • Receptors, Antigen, T-Cell
  • Receptors, Cell Surface
  • Serine Endopeptidases
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 2162887
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Additional Document Info

start page

  • 246

end page

  • 253

volume

  • 145

issue

  • 1

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