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Alteration of neural cell-adhesion molecule (n-cam) expression after neuronal cell-transformation by rous-sarcoma virus

Academic Article
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Overview

authors

  • Greenberg, M. E.
  • Brackenbury, R.
  • Edelman, Gerald

publication date

  • 1984

journal

  • Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences  Journal

abstract

  • The effect of transformation by Rous sarcoma virus on the neural cell adhesion molecule N-CAM was assessed by immunoblotting, immunofluorescence staining, and an in vitro cell-cell aggregation assay using highly specific antibodies to the adhesion molecule. Expression of N-CAM was found to be temperature dependent in several rat cerebellar cell lines infected with a mutant Rous sarcoma virus that is temperature sensitive for transformation. At the nonpermissive temperature, these cells displayed significant quantities of N-CAM and aggregated rapidly by an N-CAM-mediated mechanism. However, when the cell lines were grown at the permissive temperature, they were morphologically transformed, contained much lower amounts of N-CAM, and aggregated poorly. A similar temperature dependence of N-CAM expression was not observed in cultured primary rat cerebellar cells nor in a chemically transformed neuronal cell line. In all of the cell lines, N-CAM occurred in the adult forms; the embryonic form has so far been observed in normal embryonic tissues and a few regions of the adult brain. The findings show that N-CAM prevalence at the cell surface can be modulated by transformation with clear-cut effects on cell-cell adhesion.

subject areas

  • Animals
  • Antigens
  • Avian Sarcoma Viruses
  • Cell Adhesion Molecules
  • Cell Line
  • Cell Transformation, Neoplastic
  • Cells, Cultured
  • Cerebellum
  • Fluorescent Antibody Technique
  • Kinetics
  • Neurons
  • Rats
  • Rats, Inbred Strains
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Identity

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.81.3.969

PubMed ID

  • 6322180
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Additional Document Info

start page

  • 969

end page

  • 973

volume

  • 81

issue

  • 3

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