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Arenavirus Z-glycoprotein association requires Z myristoylation but not functional ring or late domains

Academic Article
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Overview

authors

  • Capul, A. A.
  • Perez, M.
  • Burke, E.
  • Kunz, S.
  • Buchmeier, M. J.
  • de la Torre, Juan

publication date

  • September 2007

journal

  • Journal of Virology  Journal

abstract

  • Generation of infectious arenavirus-like particles requires the virus RING finger Z protein and surface glycoprotein precursor (GPC) and the correct processing of GPC into GP1, GP2, and a stable signal peptide (SSP). Z is the driving force of arenavirus budding, whereas the GP complex (GPc), consisting of hetero-oligomers of SSP, GP1, and GP2, forms the viral envelope spikes that mediate receptor recognition and cell entry. Based on the roles played by Z and GP in the arenavirus life cycle, we hypothesized that Z and the GPc should interact in a manner required for virion formation. Here, using confocal microscopy and coimmunoprecipitation assays, we provide evidence for subcellular colocalization and biochemical interaction, respectively, of Z and the GPc. Our results from mutation-function analysis reveal that Z myristoylation, but not the Z late (L) or RING domain, is required for Z-GPc interaction. Moreover, Z interacted directly with SSP in the absence of other components of the GPc. We obtained similar results with Z and GPC from the prototypical arenavirus lymphocytic choriomeningitis virus and the hemorrhagic fever arenavirus Lassa fever virus.

subject areas

  • Animals
  • Arenavirus
  • Carrier Proteins
  • Cell Line
  • Cytoplasm
  • Glycoproteins
  • Immunoprecipitation
  • Microscopy, Confocal
  • Protein Binding
  • Protein Processing, Post-Translational
  • Viral Envelope Proteins
  • Viral Matrix Proteins
  • Viral Proteins
  • Virus Assembly
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Identity

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.00499-07

PubMed ID

  • 17581989
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Additional Document Info

start page

  • 9451

end page

  • 9460

volume

  • 81

issue

  • 17

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