Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Alternative mRNA splicing produces a novel biologically active short isoform of PGC-1 alpha

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Zhang, Y. B.
  • Huypens, P.
  • Adamson, A. W.
  • Chang, J. S.
  • Henagan, T. M.
  • Boudreau, A.
  • Lenard, N. R.
  • Burk, D.
  • Klein, J.
  • Perwitz, N.
  • Shin, J. H.
  • Fasshauer, M.
  • Kralli, Anastasia
  • Gettys, T. W.

publication date

  • November 2009

journal

  • Journal of Biological Chemistry  Journal

abstract

  • The transcriptional co-activator PGC-1alpha regulates functional plasticity in adipose tissue by linking sympathetic input to the transcriptional program of adaptive thermogenesis. We report here a novel truncated form of PGC-1alpha (NT-PGC-1alpha) produced by alternative 3' splicing that introduces an in-frame stop codon into PGC-1alpha mRNA. The expressed protein includes the first 267 amino acids of PGC-1alpha and 3 additional amino acids from the splicing insert. NT-PGC-1alpha contains the transactivation and nuclear receptor interaction domains but is missing key domains involved in nuclear localization, interaction with other transcription factors, and protein degradation. Expression and subcellular localization of NT-PGC-1alpha are dynamically regulated in the context of physiological signals that regulate full-length PGC-1alpha, but the truncated domain structure conveys unique properties with respect to protein-protein interactions, protein stability, and recruitment to target gene promoters. Therefore, NT-PGC-1alpha is a co-expressed, previously unrecognized form of PGC-1alpha with functions that are both unique from and complementary to PGC-1alpha.

subject areas

  • Adipocytes
  • Alternative Splicing
  • Animals
  • Codon, Terminator
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Protein Interaction Mapping
  • Protein Isoforms
  • RNA, Messenger
  • RNA-Binding Proteins
  • Rats
  • Rats, Inbred F344
  • Trans-Activators
  • Transcription Factors
  • Transcriptional Activation
scroll to property group menus

Identity

PubMed Central ID

  • PMC2781698

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M109.037556

PubMed ID

  • 19773550
scroll to property group menus

Additional Document Info

start page

  • 32813

end page

  • 32826

volume

  • 284

issue

  • 47

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support