Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Sequence-specific resonance assignments in the h-1-NMR spectrum of the lac repressor DNA-binding domain 1-51 from escherichia-coli by two-dimensional spectroscopy

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Zuiderweg, E. R. P.
  • Kaptein, R.
  • Wuthrich, Kurt

publication date

  • 1983

journal

  • European Journal of Biochemistry  Journal

abstract

  • The assignment of the 1H nuclear magnetic resonance (NMR) spectrum of the DNA-binding domain 1-51 of lac repressor from Escherichia coli is described and documented. The assignments are based entirely on the amino acid sequence and on two-dimensional NMR experiments at 360 MHz and 500 MHz. Individual assignments were obtained at 18 degrees C for the backbone protons of 44 out of the total of 51 amino acids residues, the exceptions being Met-1, Lys-2, Tyr-7, Arg-35, Glu-36, Lys-37 and Ile-48. Complete assignments of the non-labile hydrogen atoms of the side chain were obtained for 33 residues, and for Asn-46 and Asn-50 the delta amide protons were also identified. The chemical shifts for the assigned resonances at 18 degrees C are listed for an aqueous solution at pH 4.9 and at pH 6.8.

subject areas

  • Amino Acid Sequence
  • Amino Acids
  • Bacterial Proteins
  • Binding Sites
  • Chemical Phenomena
  • Chemistry
  • DNA
  • Escherichia coli
  • Magnetic Resonance Spectroscopy
  • Protein Binding
  • Repressor Proteins
  • Spectrum Analysis
  • Transcription Factors
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0014-2956

Digital Object Identifier (DOI)

  • 10.1111/j.1432-1033.1983.tb07827.x

PubMed ID

  • 6360686
scroll to property group menus

Additional Document Info

start page

  • 279

end page

  • 292

volume

  • 137

issue

  • 1-2

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support